Fu Xiaoxiang, Xiao Suling, Cao Duantao, Yuan Minxuan, Xiang Miaolian, Zhou Qinghong, Huang Yingjin, Wei Hongyi, Peng Wenwen
The Laboratory for Phytochemistry and Botanical Pesticides, College of Agriculture, Jiangxi Agricultural University, Nanchang, China.
Jiangxi Province Key Laboratory of Tuberous Plant Biology, Jiangxi Agricultural University, Nanchang, China.
Front Chem. 2022 Dec 13;10:1104805. doi: 10.3389/fchem.2022.1104805. eCollection 2022.
Two novel amides, named clauphenamides A and B, and twelve other known compounds were isolated from the twigs and leaves of Lour. Skeels (Rutaceae). Their structures were elucidated on the basis of extensive spectroscopic analysis and comparison with data reported in the literature. Clauphenamide A () featured in the unit of N-2-(4,8-dimethoxyfuro [2,3-b]quinolin-7-yl)vinyl, and clauphenamide B () was a unprecedented N-phenethyl cinnamide dimer. Other known compounds belong to pyrrolidone amides ( and ), furacoumarins (-), simple coumarins (-), lignan () and sesquiterpene (). Compounds , , and were separated from the genus () for the first time, while was isolated in the species () for the first time. The antifungal activities of the isolated compounds were assayed. As a result, at the concentration of 100 g/ml, compared with the control (chlorothalonil, inhibition rate of 83.67%), compounds and were found to exhibit moderate antifungal activity against with inhibition rates of 68.39% and 52.05%, respectively. Compounds - also exhibited moderate activity against and , with inhibition rates greater than 40%. In addition, compared with the control (chlorothalonil, inhibition rate of 69.02%), compounds - showed strong antifungal activity to , with inhibition rates greater than 55%. Among them, compound has the strongest antifungal activity against , and the inhibition rate (65.44%) is close to that of the control chlorothalonil. Additionally, the structure-activity relationships of the separated compounds are also discussed preliminarily in this paper.
从芸香科植物Lour. Skeels的嫩枝和叶片中分离得到了两种新型酰胺类化合物，分别命名为氯芬酰胺A和B，以及其他12种已知化合物。通过广泛的光谱分析并与文献报道的数据进行比较，阐明了它们的结构。氯芬酰胺A（）的特征结构单元为N-2-(4,8-二甲氧基呋喃并[2,3-b]喹啉-7-基)乙烯基，氯芬酰胺B（）是一种前所未有的N-苯乙基肉桂酰胺二聚体。其他已知化合物属于吡咯烷酮酰胺（和）、呋喃香豆素（-）、简单香豆素（-）、木脂素（）和倍半萜（）。化合物、、和首次从属（）中分离得到，而首次从种（）中分离得到。对分离得到的化合物进行了抗真菌活性测定。结果表明，在浓度为100 μg/ml时，与对照（百菌清，抑制率为83.67%）相比，化合物和对表现出中等抗真菌活性，抑制率分别为68.39%和52.05%。化合物-对和也表现出中等活性，抑制率大于40%。此外，与对照（百菌清，抑制率为69.02%）相比，化合物-对表现出较强的抗真菌活性，抑制率大于55%。其中，化合物对的抗真菌活性最强，抑制率（65.44%）接近对照百菌清。此外，本文还初步讨论了分离得到的化合物的构效关系。
