Goluch Edgar D, Wolfrum Bernhard, Singh Pradyumna S, Zevenbergen Marcel A G, Lemay Serge G
Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ, Delft, The Netherlands.
Anal Bioanal Chem. 2009 May;394(2):447-56. doi: 10.1007/s00216-008-2575-x. Epub 2009 Jan 6.
Amperometric detection is ideally suited for integration into micro- and nanofluidic systems as it directly yields an electrical signal and does not necessitate optical components. However, the range of systems to which it can be applied is constrained by the limited sensitivity and specificity of the method. These limitations can be partially alleviated through the use of redox cycling, in which multiple electrodes are employed to repeatedly reduce and oxidize analyte molecules and thereby amplify the detected signal. We have developed an interdigitated electrode device that is encased in a nanofluidic channel to provide a hundred-fold amplification of the amperometric signal from paracetamol. Due to the nanochannel design, the sensor is resistant to interference from molecules undergoing irreversible redox reactions. We demonstrate this selectivity by detecting paracetamol in the presence of excess ascorbic acid.
安培检测非常适合集成到微纳流体系统中，因为它直接产生电信号，不需要光学组件。然而，该方法可应用的系统范围受到其有限的灵敏度和特异性的限制。通过使用氧化还原循环可以部分缓解这些限制，其中使用多个电极反复还原和氧化分析物分子，从而放大检测信号。我们开发了一种叉指电极装置，该装置封装在纳米流体通道中，可将对乙酰氨基酚的安培信号放大一百倍。由于纳米通道设计，该传感器对经历不可逆氧化还原反应的分子的干扰具有抗性。我们通过在过量抗坏血酸存在的情况下检测对乙酰氨基酚来证明这种选择性。
