Moon Hee Jung, Lee Sang Ho, Ku Mi Jeong, Yu Byeng Chul, Jeon Man Joong, Jeong Seok Hoon, Stonik Valentin A, Zvyagintseva Tatyana N, Ermakova Svetlana P, Lee Yong Hwan
Institute of Natural Products for Health Promotion and Department of Preventive Medicine, College of Medicine, Kosin University, 34 Amnam-dong, Suh-gu, Busan, 602-702, Republic of Korea.
Eur J Dermatol. 2009 Mar-Apr;19(2):129-34. doi: 10.1684/ejd.2008.0611. Epub 2009 Jan 7.
UVB reduces type I procollagen levels and increases matrix metalloproteinases-1 (MMP-1) levels in human skin and plays a major role in the process of photoaging. We previously reported that fucoidan inhibits UVB-induced MMP-1 expression at the protein and mRNA levels in human skin fibroblasts (HS68). Yet, the effects of fucoidan on UVB-induced MMP-1 promoter activity and type I procollagen have not been investigated. In this study, we assessed the effects of fucoidan on the inhibition of MMP-1 promoter activity and on the increase of type I procollagen synthesis in human skin fibroblasts. Fucoidan treatment significantly inhibited MMP-1 promoter activity compared to UVB irradiation alone. Fucoidan treatment also increased type I procollagen mRNA and protein expression in a dose-dependent manner compared to the control. Our data indicate that fucoidan may prevent UVB-induced MMP-1 expression and inhibit down-regulation of type I procollagen synthesis. We suggest that fucoidan may be a potential therapeutic agent to prevent and treat skin photoaging.
紫外线B（UVB）可降低人体皮肤中I型前胶原水平，并提高基质金属蛋白酶-1（MMP-1）水平，在光老化过程中起主要作用。我们之前报道过，岩藻依聚糖在蛋白质和mRNA水平上抑制人皮肤成纤维细胞（HS68）中UVB诱导的MMP-1表达。然而，岩藻依聚糖对UVB诱导的MMP-1启动子活性和I型前胶原的影响尚未得到研究。在本研究中，我们评估了岩藻依聚糖对人皮肤成纤维细胞中MMP-1启动子活性抑制以及I型前胶原合成增加的影响。与单独UVB照射相比，岩藻依聚糖处理显著抑制了MMP-1启动子活性。与对照组相比，岩藻依聚糖处理还以剂量依赖的方式增加了I型前胶原mRNA和蛋白质表达。我们的数据表明，岩藻依聚糖可能预防UVB诱导的MMP-1表达，并抑制I型前胶原合成的下调。我们认为岩藻依聚糖可能是预防和治疗皮肤光老化的潜在治疗剂。
