Choi Hee-Jeong, Alam Md Badrul, Baek Mi-Eun, Kwon Yoon-Gyung, Lim Ji-Young, Lee Sang-Han
Department of Food Science And Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Republic of Korea.
Food and Bio-Industry Research Institute, Inner Beauty/Antiaging Center, Kyungpook National University, Daegu 41566, Republic of Korea.
Oxid Med Cell Longev. 2020 Jun 22;2020:2905362. doi: 10.1155/2020/2905362. eCollection 2020.
Ultraviolet B (UVB) irradiation is major causative factor in skin aging. The aim of the present study was to investigate the protective effect of a 50% ethanol extract from (NF50E) against UVB-induced skin aging. The results indicated that NF50E exerted potent antioxidant activity (IC = 17.55 ± 1.63 and 10.78 ± 0.63 g/mL for DPPH and ABTS-radical scavenging activity, respectively) in a dose-dependent manner. High-performance liquid chromatography revealed that pengxianencin A, protocatechuic acid, catechin, chlorogenic acid, epicatechin, and kaempferol were components of the extract. In addition, the extract exhibited elastase inhibitory activity (IC = 17.96 ± 0.39 g/mL). NF50E protected against UVB-induced HaCaT cell death and strongly suppressed UVB-stimulated cellular reactive oxygen species generation without cellular toxicity. Moreover, topical application of NF50E mitigated UVB-induced photoaging lesions including skin erythema and skin thickness in BALB/C mice. NF50E treatment inhibited UVB-induced collagen degradation as well as MMP-1 and IL-1 expressions and significantly stimulated SIRT1 expression. Furthermore, the extract treatment markedly suppressed the activation of NF-B and AP-1 (p-c-Jun) by deactivating the p38 and JNK proteins. Taken together, current data suggest that NF50E exhibits potent antioxidant potential and protection against photoaging by attenuating MMP-1 activity and collagen degradation possibly through the downregulation of MAPK/NF-B/AP-1 signaling and SIRT1 activation.
紫外线B（UVB）照射是皮肤衰老的主要致病因素。本研究的目的是探讨[具体植物名称]50%乙醇提取物（NF50E）对UVB诱导的皮肤衰老的保护作用。结果表明，NF50E以剂量依赖的方式发挥强大的抗氧化活性（DPPH和ABTS自由基清除活性的IC50分别为17.55±1.63和10.78±0.63μg/mL）。高效液相色谱显示，彭县霉素A、原儿茶酸、儿茶素、绿原酸、表儿茶素和山奈酚是该提取物的成分。此外，该提取物表现出弹性蛋白酶抑制活性（IC50=17.96±0.39μg/mL）。NF50E可保护HaCaT细胞免受UVB诱导的死亡，并强烈抑制UVB刺激的细胞活性氧生成，且无细胞毒性。此外，在BALB/C小鼠中局部应用NF50E可减轻UVB诱导的光老化损伤，包括皮肤红斑和皮肤厚度。NF50E处理可抑制UVB诱导的胶原蛋白降解以及MMP-1和IL-1的表达，并显著刺激SIRT1的表达。此外，提取物处理通过使p38和JNK蛋白失活，显著抑制了NF-κB和AP-1（p-c-Jun）的激活。综上所述，目前的数据表明，NF50E具有强大的抗氧化潜力，并通过下调MAPK/NF-κB/AP-1信号通路和激活SIRT1，可能通过减弱MMP-1活性和胶原蛋白降解来保护皮肤免受光老化。
