Palecek E
Max-Planck Institut für Biophysikalische Chemie, Göttingen, BRD.
Crit Rev Biochem Mol Biol. 1991;26(2):151-226. doi: 10.3109/10409239109081126.
The DNA double helix exhibits local sequence-dependent polymorphism at the level of the single base pair and dinucleotide step. Curvature of the DNA molecule occurs in DNA regions with a specific type of nucleotide sequence periodicities. Negative supercoiling induces in vitro local nucleotide sequence-dependent DNA structures such as cruciforms, left-handed DNA, multistranded structures, etc. Techniques based on chemical probes have been proposed that make it possible to study DNA local structures in cells. Recent results suggest that the local DNA structures observed in vitro exist in the cell, but their occurrence and structural details are dependent on the DNA superhelical density in the cell and can be related to some cellular processes.
DNA双螺旋在单碱基对和二核苷酸步长水平上表现出局部序列依赖性多态性。DNA分子的弯曲发生在具有特定类型核苷酸序列周期性的DNA区域。负超螺旋在体外诱导局部核苷酸序列依赖性DNA结构，如十字形、左手DNA、多链结构等。已经提出了基于化学探针的技术，这些技术使得研究细胞中的DNA局部结构成为可能。最近的结果表明，在体外观察到的局部DNA结构存在于细胞中，但其出现和结构细节取决于细胞中的DNA超螺旋密度，并且可能与一些细胞过程有关。
