Everard C O, Baulu J, Carrington D G, Korver H, Terpstra W J
Leptospira Laboratory, St. Michael, Barbados.
Eur J Epidemiol. 1991 Jul;7(4):396-402. doi: 10.1007/BF00145006.
In a study of 21 wild-caught Barbadian vervet monkeys (Cercopithecus aethiops sabaeus), naturally-acquired leptospiral agglutinins were found to persist for over five years. Groups of seropositive as well as seronegative vervets were given a placebo, or full-strength monoclonal antibodies MCA F12C3 (Icterohaemorrhagiae copenhageni), or diluted F12C3 MCAs. They were challenged 24 hours later with a suspension of highly virulent leptospires (copenhageni) administered intraperitoneally. Immunoprotection was evident in animals receiving full strength MCAs as measured by their failure to develop any substantial antibody response and by their lower geometric mean titres over a period of 142 weeks (maximum GMT of 113 compared with a maximum of 1280 in the placebo group). Diluted MCAs had little or no protective value. The serological response of the monkeys which were seropositive at capture to challenge with virulent copenhageni antigen was strongly anamnestic both in those given MCAs and those given placebo. None of the naturally or experimentally infected vervets showed clinical signs of leptospiral illness.
在一项对21只野生捕获的巴巴多斯绿猴（Cercopithecus aethiops sabaeus）的研究中，发现自然获得的钩端螺旋体凝集素可持续五年以上。给血清阳性和血清阴性的绿猴组分别给予安慰剂、全强度单克隆抗体MCA F12C3（哥本哈根出血黄疸型）或稀释的F12C3单克隆抗体。24小时后，给它们腹腔注射高毒力钩端螺旋体（哥本哈根型）悬液进行攻毒。通过观察动物在142周内未产生任何明显的抗体反应以及较低的几何平均滴度（最高几何平均滴度为113，而安慰剂组最高为1280），可以看出接受全强度单克隆抗体的动物具有免疫保护作用。稀释的单克隆抗体几乎没有或没有保护价值。捕获时血清阳性的猴子在用强毒哥本哈根抗原攻毒后的血清学反应，在给予单克隆抗体和给予安慰剂的猴子中均强烈表现为回忆反应。无论是自然感染还是实验感染的绿猴，均未表现出钩端螺旋体病临床症状。
