Sadjjadi Seyed Mahmoud, Zoharizadeh Mohammad Reza, Panjeshahin Mohammad Reza
Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
J Invest Surg. 2008 Nov-Dec;21(6):318-22. doi: 10.1080/08941930802348261.
This study was conducted to assess the protoscolicidal effect of garlic (Allium sativum) extract on protoscoleces of hydatid cysts in vitro. Viability of protoscoleces was determined, as the percent of viable protoscoleces to the total protoscoleces in 1 ml of the hydatid fluid, using eosin stain method. Four types of garlic extracts including: aquaus extract, chloroform extract (with two concentrations) and hydro-alcoholic extract along with cetrimide and silver nitrate as positive control, and normal saline as negative control, were used in this study. In vitro tests were performed at 37 degrees C with exposure times of 30 minutes, one, two, four, and six hours. The protoscolecidal activity of the used materials was determined by counting of 250 protoscoleces. The mean percent and standard deviation (SD) of protoscolecidal activity of different used materials were as follows: cetrimide: 100 +/- 00, garlic chloroform extract with 200 mg/ml concentration: 99.58 +/- 1.63, garlic chloroform extract with 100 mg/ml concentration: 96.06 +/- 7.28, silver nitrate: 95.91 +/- 7.11, garlic hydro-alcoholic extract: 59.82 +/- 21.48, garlic simple extract: 40.91 +/- 18.1, and of saline: 16.01 +/- 6.25. The protoscolecidal activity increased by the time of exposure. Results of this study showed that chloroform extract of garlic with 200 mg/ml concentration have the highest and simple extract of garlic have the lowest protoscolecidal activity.
本研究旨在体外评估大蒜（葱属植物）提取物对棘球蚴原头节的杀灭作用。使用伊红染色法，以每毫升棘球蚴液中存活原头节占原头节总数的百分比来确定原头节的活力。本研究使用了四种类型的大蒜提取物，包括：水提取物、氯仿提取物（两种浓度）和水醇提取物，同时使用西曲溴铵和硝酸银作为阳性对照，生理盐水作为阴性对照。体外试验在37℃下进行，暴露时间分别为30分钟、1小时、2小时、4小时和6小时。通过计数250个原头节来测定所用材料的杀原头节活性。不同所用材料杀原头节活性的平均百分比和标准差（SD）如下：西曲溴铵：100±00，浓度为200mg/ml的大蒜氯仿提取物：99.58±1.63，浓度为100mg/ml的大蒜氯仿提取物：96.06±7.28，硝酸银：95.91±7.11，大蒜水醇提取物：59.82±21.48，大蒜单纯提取物：40.91±18.1，生理盐水：16.01±6.25。杀原头节活性随暴露时间增加。本研究结果表明，浓度为200mg/ml的大蒜氯仿提取物杀原头节活性最高，大蒜单纯提取物杀原头节活性最低。
