提取物和二氧化铈纳米颗粒对包虫囊肿的体外和体内杀包虫活性

In Vitro and in Vivo Scolicidal Activities of Extract and CeO2 Nanoparticles against Hydatid Cyst.

作者信息

Aryamand Safa, Khademvatan Shahram, Hazrati Tappeh Khosrow, Heshmatian Behnam, Jelodar Ali

机构信息

Cellular and Molecular Research Center, Department of Medical Parasitology and Mycology, Urmia University of Medical Sciences, Urmia, Iran.

Department of Physiology, Urmia University of Medical Sciences, Urmia, Iran.

出版信息

Iran J Parasitol. 2019 Apr-Jun;14(2):269-279.

BACKGROUND

We aimed to investigate the scolicidal effects of extract and CeO2 nanoparticles against protoscoleces of hydatid cysts in-vitro and in-vivo.

METHODS

Hydatid cysts were collected from, Urmia slaughterhouses between years 2016-2017 and the hydatid fluid aspirated from the fertile cysts. Various concentration of extract, CeO2 NPs and combination of CeO2-NPs/ were used for 10-60 min to evaluate the viability of protoscoleces by 0.1% eosin method. CASPASE -3 activity measured for assessment of cell apoptosis in treated protoscoleces. BALB/c mice were infected intraperitoneally with 2000 viable protoscoleces and treated daily for 4 wk by intragastrical inoculation with , CeO2 NPs, combination of CeO2 NPs/ and Albendazole. Cyst development was macroscopically analyzed.

RESULTS

extract and combination of CeO2 NPs/ have potent scolicidal activity at concentration of 20 mg/ml and 15 mg/ml after 60 min treatment. Maximum caspase-3 activity was observed when protoscoleces expose with and combination & CeO2 NPs. After treatment of cyst infected mice with extract and CeO2 NPs, combination of CeO2 NPs/ and albendazole, a significant decrease in number of cysts, size and volume of cyst (<0.05) was observed.

CONCLUSION

This result shows an antihydatic and scolicidal effects of extract and CeO2 .

背景

我们旨在研究[提取物名称]提取物和二氧化铈纳米颗粒在体外和体内对包虫囊肿原头节的杀头节作用。

方法

2016 - 2017年间从乌尔米耶屠宰场收集包虫囊肿，并从有活力的囊肿中抽取囊液。使用不同浓度的[提取物名称]提取物、二氧化铈纳米颗粒以及二氧化铈纳米颗粒/[提取物名称]的组合处理10 - 60分钟，通过0.1%伊红法评估原头节的活力。检测半胱天冬酶 - 3活性以评估处理后原头节的细胞凋亡情况。将2000个有活力的原头节腹腔注射感染BALB/c小鼠，并通过灌胃接种[提取物名称]、二氧化铈纳米颗粒、二氧化铈纳米颗粒/[提取物名称]的组合以及阿苯达唑，每日处理4周。对囊肿发育进行宏观分析。

结果

[提取物名称]提取物以及二氧化铈纳米颗粒/[提取物名称]的组合在60分钟处理后，浓度为20毫克/毫升和15毫克/毫升时具有强大的杀头节活性。当原头节暴露于[提取物名称]以及[提取物名称]与二氧化铈纳米颗粒的组合时，观察到最大半胱天冬酶 - 3活性。在用[提取物名称]提取物、二氧化铈纳米颗粒、二氧化铈纳米颗粒/[提取物名称]的组合以及阿苯达唑处理感染囊肿的小鼠后，观察到囊肿数量、大小和体积显著减少（<0.05）。