Murawaki Y, Kato S, Hirayama C
Second Department of Internal Medicine, Tottori University School of Medicine, Yonago, Japan.
Gastroenterol Jpn. 1991 Aug;26(4):465-71. doi: 10.1007/BF02782815.
To examine the synthesis of hepatic collagen in patients with alcoholic and nonalcoholic liver disease, liver biopsy specimens were incubated in vitro with 14C-proline, and the radioactivity of the newly synthesized protein-bound 14C-hydroxyproline was measured. Mean hepatic collagen synthesis was 0.82 +/- 0.19 pmole of 14C-hydroxyproline/g liver/2 h in control subjects without histological liver fibrosis. Hepatic collagen synthesis was increased in patients with alcoholic and nonalcoholic liver diseases, especially in those with alcoholic fibrosis, alcoholic cirrhosis and chronic active hepatitis. The raised collagen synthesis in alcoholic liver disease rapidly decreased after withdrawal of alcohol. When alcoholic liver disease were compared with nonalcoholic liver disease, there was no significant difference in hepatic collagen synthesis.
为研究酒精性肝病和非酒精性肝病患者肝胶原的合成情况，将肝活检标本与14C - 脯氨酸进行体外孵育，然后测定新合成的蛋白结合型14C - 羟脯氨酸的放射性。在无组织学肝纤维化的对照受试者中，平均肝胶原合成量为0.82±0.19皮摩尔14C - 羟脯氨酸/克肝脏/2小时。酒精性肝病和非酒精性肝病患者的肝胶原合成增加，尤其是酒精性纤维化、酒精性肝硬化和慢性活动性肝炎患者。戒酒之后，酒精性肝病患者升高的胶原合成迅速下降。当比较酒精性肝病和非酒精性肝病时，肝胶原合成无显著差异。
