Brazas R, Davie E, Farewell A, Rothfield L I
Department of Microbiology, University of Connecticut Health Center, Farmington 06032.
J Bacteriol. 1991 Oct;173(19):6168-73. doi: 10.1128/jb.173.19.6168-6173.1991.
The transcriptional organization of the rfaGBIJ gene cluster of Salmonella typhimurium was studied by using lacZ and cat transcriptional probes. The results indicated that the leftward end of the gene cluster (rfaG-rfaB-rfaI) is an operon that is transcribed from one or more promoters that lie upstream of rfaG. The results further indicated that the product of the rfaH (sfrB) gene acts as a positive regulator of transcription of the entire rfaGBIJ cluster. At least one site required for the RfaH-mediated transcriptional regulation lies within or very close to the upstream promoter.
利用lacZ和cat转录探针研究了鼠伤寒沙门氏菌rfaGBIJ基因簇的转录组织。结果表明,基因簇的左端(rfaG - rfaB - rfaI)是一个操纵子,它从位于rfaG上游的一个或多个启动子转录。结果还表明,rfaH(sfrB)基因的产物作为整个rfaGBIJ簇转录的正调控因子。RfaH介导的转录调控所需的至少一个位点位于上游启动子内或非常靠近上游启动子。