嗜碱芽孢杆菌菌株170的脂青霉素酶基因的核苷酸序列。

The nucleotide sequence of the lipo-penicillinase gene of alkalophilic Bacillus sp. strain 170.

作者信息

Kato C, Nakano Y, Horikoshi K

机构信息

Laboratory of Microbiol Metabolism, Research Development Corporation of Japan, Tokyo.

出版信息

Arch Microbiol. 1989;151(2):91-4. doi: 10.1007/BF00414419.

DOI:10.1007/BF00414419

PMID:2655551

Abstract

The lipo-penicillinase (LIPEN) gene from an alkalophilic Bacillus sp. strain 170 was cloned in Escherichia coli using the vector pHSG399. A plasmid, pFAP121, was isolated from an ampicillin resistant transformant and the cloned LIPEN gene was found to be in a 2.2 kb DNA fragment. The nucleotide sequence of a 1.9 kb segment encoding the LIPEN was determined. This segment showed an open reading frame which would encode a polypeptide of 310 amino acids. The amino acid sequence of this LIPEN gene product has strong homology with those of the Bacillus cereus beta-lactamase III and Bacillus licheniformis penicillinase.

摘要

利用载体pHSG399，将嗜碱芽孢杆菌菌株170的脂溶性青霉素酶（LIPEN）基因克隆到大肠杆菌中。从氨苄青霉素抗性转化体中分离出一个质粒pFAP121，发现克隆的LIPEN基因位于一个2.2 kb的DNA片段中。测定了编码LIPEN的1.9 kb片段的核苷酸序列。该片段显示出一个开放阅读框，可编码一个由310个氨基酸组成的多肽。该LIPEN基因产物的氨基酸序列与蜡样芽孢杆菌β-内酰胺酶III和地衣芽孢杆菌青霉素酶的氨基酸序列具有高度同源性。