Frank J, Verschoor A, Boublik M
Science. 1981 Dec 18;214(4527):1353-5. doi: 10.1126/science.7313694.
An enhanced lateral view of the 40S ribosomal subunit of HeLa cells has been obtained by computer averaging of single particles visualized in the electron microscope. Application of crystallographic criteria to independent averages shows that the reproducibility of the result is comparable to that obtained for thin, stained protein crystals by conventional Fourier filtration methods.
通过对电子显微镜下可视化的单个颗粒进行计算机平均处理，获得了HeLa细胞40S核糖体亚基的增强侧视图。将晶体学标准应用于独立平均值表明，结果的可重复性与通过传统傅里叶滤波方法对薄的、染色的蛋白质晶体所获得的可重复性相当。
