Ballhausen Britta, Altendorf Karlheinz, Deckers-Hebestreit Gabriele
Arbeitsgruppe Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück, Germany.
J Bacteriol. 2009 Apr;191(7):2400-4. doi: 10.1128/JB.01390-08. Epub 2009 Jan 30.
The subunit c stoichiometry of Escherichia coli ATP synthase was studied by intermolecular cross-linking via oxidation of bi-cysteine-substituted subunit c (cA21C/cM65C). Independent of the carbon source used for growth and independent of the presence of other FoF1 subunits, an equal pattern of cross-link formation stopping at the formation of decamers was obtained.
通过对双半胱氨酸取代的亚基c(cA21C/cM65C)进行氧化,利用分子间交联的方法研究了大肠杆菌ATP合酶的亚基c化学计量。无论用于生长的碳源如何,也无论其他F₀F₁亚基是否存在,均获得了一种相同的交联形成模式,该模式在十聚体形成时停止。