Wildt Bridget, Wirtz Denis, Searson Peter C
Department of Materials Science and Engineering, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA.
Nat Methods. 2009 Mar;6(3):211-3. doi: 10.1038/nmeth.1299. Epub 2009 Feb 1.
Cell detachment is central to a broad range of physiopathological changes, but there are no quantitative methods to study this process. Here we report programmed subcellular release, a method for spatially and temporally controlled cellular detachment, and present quantitative results of the detachment dynamics of 3T3 fibroblasts at the subcellular level.
细胞脱离是广泛的生理病理变化的核心,但目前尚无定量方法来研究这一过程。在此,我们报告了程序性亚细胞释放,这是一种用于在空间和时间上控制细胞脱离的方法,并展示了3T3成纤维细胞在亚细胞水平上脱离动力学的定量结果。