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利用阵列比较基因组杂交技术对秀丽隐杆线虫单核苷酸突变进行从头鉴定。

De Novo identification of single nucleotide mutations in Caenorhabditis elegans using array comparative genomic hybridization.

作者信息

Maydan Jason S, Okada H Mark, Flibotte Stephane, Edgley Mark L, Moerman Donald G

机构信息

Department of Zoology, University of British Columbia V6T 1Z4, Vancouver, British Columbia, Canada.

出版信息

Genetics. 2009 Apr;181(4):1673-7. doi: 10.1534/genetics.108.100065. Epub 2009 Feb 2.

DOI:10.1534/genetics.108.100065
PMID:19189945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2666529/
Abstract

Array comparative genomic hybridization (aCGH) has been used primarily to detect copy-number variants between two genomes. Here we report using aCGH to detect single nucleotide mutations on oligonucleotide microarrays with overlapping 50-mer probes. This technique represents a powerful method for rapidly detecting novel homozygous single nucleotide mutations in any organism with a sequenced reference genome.

摘要

阵列比较基因组杂交（aCGH）主要用于检测两个基因组之间的拷贝数变异。在此，我们报告使用aCGH在具有重叠50聚体探针的寡核苷酸微阵列上检测单核苷酸突变。该技术是一种强大的方法，可用于在任何具有已测序参考基因组的生物体中快速检测新的纯合单核苷酸突变。

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Genetics. 2009 Apr;181(4):1673-7. doi: 10.1534/genetics.108.100065. Epub 2009 Feb 2.
2
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本文引用的文献

1
Cis-regulatory mutations in the Caenorhabditis elegans homeobox gene locus cog-1 affect neuronal development.秀丽隐杆线虫同源异型框基因座cog-1中的顺式调控突变影响神经元发育。
Genetics. 2009 Apr;181(4):1679-86. doi: 10.1534/genetics.108.097832. Epub 2009 Feb 2.
2
Rapid high resolution single nucleotide polymorphism-comparative genome hybridization mapping in Caenorhabditis elegans.秀丽隐杆线虫中快速高分辨率单核苷酸多态性-比较基因组杂交图谱分析
Genetics. 2009 Jan;181(1):33-7. doi: 10.1534/genetics.108.096487. Epub 2008 Oct 28.
3
Experimental analysis of oligonucleotide microarray design criteria to detect deletions by comparative genomic hybridization.用于通过比较基因组杂交检测缺失的寡核苷酸微阵列设计标准的实验分析。
BMC Genomics. 2008 Oct 21;9:497. doi: 10.1186/1471-2164-9-497.
4
Mapping short DNA sequencing reads and calling variants using mapping quality scores.使用比对质量分数比对短DNA测序读数并识别变异。
Genome Res. 2008 Nov;18(11):1851-8. doi: 10.1101/gr.078212.108. Epub 2008 Aug 19.
5
Caenorhabditis elegans mutant allele identification by whole-genome sequencing.通过全基因组测序鉴定秀丽隐杆线虫突变等位基因
Nat Methods. 2008 Oct;5(10):865-7. doi: 10.1038/nmeth.1249. Epub 2008 Aug 1.
6
A study of the relationships between oligonucleotide properties and hybridization signal intensities from NimbleGen microarray datasets.一项关于寡核苷酸特性与来自NimbleGen微阵列数据集的杂交信号强度之间关系的研究。
Nucleic Acids Res. 2008 May;36(9):2926-38. doi: 10.1093/nar/gkn133. Epub 2008 Apr 1.
7
Comparing whole genomes using DNA microarrays.使用DNA微阵列比较全基因组。
Nat Rev Genet. 2008 Apr;9(4):291-302. doi: 10.1038/nrg2335.
8
Whole-genome sequencing and variant discovery in C. elegans.秀丽隐杆线虫的全基因组测序与变异发现
Nat Methods. 2008 Feb;5(2):183-8. doi: 10.1038/nmeth.1179. Epub 2008 Jan 20.
9
Optimal design of oligonucleotide microarrays for measurement of DNA copy-number.用于测量DNA拷贝数的寡核苷酸微阵列的优化设计。
Hum Mol Genet. 2007 Nov 15;16(22):2770-9. doi: 10.1093/hmg/ddm234. Epub 2007 Aug 28.
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Efficient target-selected mutagenesis in Caenorhabditis elegans: toward a knockout for every gene.秀丽隐杆线虫中高效的靶向选择诱变：实现每个基因的敲除
Genome Res. 2007 May;17(5):649-58. doi: 10.1101/gr.6080607. Epub 2007 Apr 6.