Sarin Sumeet, Prabhu Snehit, O'Meara M Maggie, Pe'er Itsik, Hobert Oliver
Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University Medical Center, New York, New York 10032, USA.
Nat Methods. 2008 Oct;5(10):865-7. doi: 10.1038/nmeth.1249. Epub 2008 Aug 1.
Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.
通过正向遗传筛选分离出的秀丽隐杆线虫突变体中分子损伤的鉴定通常涉及耗时的遗传定位。我们使用Illumina深度测序技术对完整的突变秀丽隐杆线虫基因组进行测序,从而在基因组中精确找到了一个影响神经元细胞命运决定的单核苷酸突变。这构成了使用全基因组测序分析秀丽隐杆线虫突变体的原理验证。