Steed L L, Setareh M, Friedman R L
Department of Microbiology and Immunology, University of Arizona, Tucson.
J Leukoc Biol. 1991 Oct;50(4):321-30. doi: 10.1002/jlb.50.4.321.
Little is known regarding the interaction of Bordetella pertussis with polymorphonuclear leukocytes (PMNL) or the role PMNL play as an initial line of defense against B. pertussis infection. An in vitro system was developed to establish conditions for the study of phagocytosis and killing of virulent B. pertussis by human PMNL. Phagocytosis of B. pertussis strains BP504, BP165, and BP338 occurred by opsonization with anti-B. pertussis antibody, while autologous normal human sera did not induce significant phagocytosis. In PMNL bacterial killing assays virulent B. pertussis strains survived PMNL bactericidal activities while Escherichia coli controls were readily killed. Electron microscopy studies using acid phosphatase as a lysosomal marker strongly suggested that B. pertussis inhibits phagosome-lysosome fusion in PMNL. These results indicate that virulent B. pertussis strains are capable of surviving intracellularly within PMNL and that such survival may be due to inhibition of phagosome-lysosome fusion.
关于百日咳博德特氏菌与多形核白细胞（PMNL）的相互作用，以及PMNL作为抵御百日咳博德特氏菌感染的第一道防线所起的作用，目前所知甚少。我们开发了一种体外系统，以建立研究人类PMNL对有毒力的百日咳博德特氏菌进行吞噬和杀伤的条件。百日咳博德特氏菌菌株BP504、BP165和BP338通过用抗百日咳博德特氏菌抗体进行调理作用而发生吞噬，而自体正常人血清未诱导显著的吞噬作用。在PMNL细菌杀伤试验中，有毒力的百日咳博德特氏菌菌株在PMNL的杀菌活性中存活下来，而大肠杆菌对照则很容易被杀死。使用酸性磷酸酶作为溶酶体标记的电子显微镜研究强烈表明，百日咳博德特氏菌抑制PMNL中吞噬体-溶酶体融合。这些结果表明，有毒力的百日咳博德特氏菌能够在PMNL内细胞内存活，并且这种存活可能是由于吞噬体-溶酶体融合受到抑制。
