Su Huanxing, Zhang Wenming, Guo Jiasong, Guo Anchen, Yuan Qiuju, Wu Wutian
Department of Anatomy, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pokfulam, Hong Kong SAR, China.
J Neurotrauma. 2009 Jan;26(1):67-80. doi: 10.1089/neu.2008.0656.
In the present study, we transplanted E13.5 spinal cord-derived neural progenitor cells (NPCs) into the acutely avulsed ventral horn of adult rats. The results showed that NPCs survived and integrated nicely within the host ventral horn at 6 weeks post-grafting. Although the majority of grafted NPCs differentiated into astrocytes and only a small proportion into neuronal cells, interestingly, grafted NPCs in the avulsed ventral horn significantly enhanced the survival of injured motoneurons and promoted their regeneration into a peripheral nerve (PN) graft, as revealed by retrograde FluoroGold (FG) labeling. Specific ELISAs, Western blotting, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that NPCs produced nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and glial cell line-derived neutrophilic factor (GDNF), both in vitro and after transplantation in vivo. These results indicate that NPCs have beneficial effects on the survival and axonal regeneration of avulsion-injured motoneurons after transplantation. Such beneficial effects are possibly due to their inherent ability to secrete various trophic factors after transplantation in vivo.
在本研究中，我们将E13.5脊髓源性神经祖细胞（NPCs）移植到成年大鼠急性撕脱的腹角。结果显示，移植后6周时，NPCs在宿主腹角内存活并良好整合。尽管大多数移植的NPCs分化为星形胶质细胞，只有一小部分分化为神经元细胞，但有趣的是，如逆行荧光金（FG）标记所示，撕脱腹角中的移植NPCs显著提高了受损运动神经元的存活率，并促进它们向周围神经（PN）移植物中再生。特异性酶联免疫吸附测定（ELISA）、蛋白质印迹法和定量实时逆转录聚合酶链反应（RT-PCR）表明，NPCs在体外和体内移植后均产生神经生长因子（NGF）、脑源性神经营养因子（BDNF）和胶质细胞系源性神经营养因子（GDNF）。这些结果表明，NPCs对移植后撕脱损伤的运动神经元的存活和轴突再生具有有益作用。这种有益作用可能归因于它们在体内移植后分泌各种营养因子的内在能力。
