Dall'Asta Chiara, Galaverna Gianni, Mangia Mattia, Sforza Stefano, Dossena Arnaldo, Marchelli Rosangela
Dipartimento di Chimica Organica e Industriale, Università di Parma, Parma, Italy.
Mol Nutr Food Res. 2009 Apr;53(4):492-9. doi: 10.1002/mnfr.200800088.
In this work a multiresidual LC-ESI-MS/MS method for the simultaneous detection of free and bound fumonisins is described, which allowed for a very low LOD and a very good recovery for all the analytes. The method was applied to the determination of free and bound fumonisins in several gluten-free products from the Italian market. Free fumonisins were found to occur in 90% of the samples: the overall median value was below the EU legal limit for foods for human consumption (800 microg/kg). Nonetheless, fumonisins occurred in several samples at concentrations above the legal limit, reaching also very strong contamination levels (maximum concentration level: 3310 microg/kg). Anyway, considering the limited diet of people suffering of the celiac disease or allergic to other wheat proteins, the incidence of fumonisin contamination may be envisaged as problematic. Furthermore, bound fumonisins were found to be present in all the analysed samples at similar or even higher amounts than the free forms. In many cases the sum of free and bound fumonisins exceeded the EU legal limit for total fumonisins also for those samples characterized by a low contamination of free fumonisins, thus opening a new important task to be addressed for the risk assessment in this field.
在本研究中，描述了一种用于同时检测游离和结合伏马毒素的多残留液相色谱 - 电喷雾串联质谱法，该方法对所有分析物具有极低的检测限和良好的回收率。该方法应用于测定意大利市场上几种无麸质产品中的游离和结合伏马毒素。发现90%的样品中存在游离伏马毒素：总体中位数低于欧盟人类食用食品的法定限量（800微克/千克）。尽管如此，仍有几个样品中的伏马毒素浓度超过法定限量，甚至达到了非常高的污染水平（最高浓度水平：3310微克/千克）。无论如何，考虑到患有乳糜泻或对其他小麦蛋白过敏的人的饮食有限，伏马毒素污染的发生率可能被认为是有问题的。此外，发现所有分析样品中结合伏马毒素的含量与游离形式相似甚至更高。在许多情况下，对于那些游离伏马毒素污染较低的样品，游离和结合伏马毒素的总和也超过了欧盟总伏马毒素的法定限量，因此为该领域的风险评估提出了一项新的重要任务。
