Mir Sajad Hussain, Darzi Mohd Maqbool
Postgraduate Department of Zoology, University of Kashmir, Srinagar, Jammu and Kashmir, India.
Int J Exp Pathol. 2009 Feb;90(1):66-73. doi: 10.1111/j.1365-2613.2008.00615.x.
The objective of this study was to investigate the prolonged complications of untreated diabetes on histomorphology of rabbits. Diabetes mellitus was experimentally induced in one group of New Zealand white male rabbits by intraperitoneal administration of four doses of alloxan @ 80 mg/kg b.w. at weekly intervals following 12 h fasting. Other group of rabbits served as healthy controls that received isotonic saline in a similar manner. The establishment of diabetes mellitus was confirmed by fasting blood glucose levels. For histomorphological study of different organs, 50% of the animals were killed after 7 weeks and the rest after 26 weeks. Routine haematoxylin and eosin stain and Gomori's modified stain were used. The blood glucose level of diabetic rabbits increased significantly throughout the experimental period. The peak values for blood sugar were on the sixth week of the study. Further, histomorphological alterations were recorded in pancreas, kidneys, lungs, heart and brain in diabetic rabbits. However, mild changes were observed in gastrointestinal tract with proliferation of yeasts in the stomach. With the progress of untreated diabetes, the histoanatomical alterations intensify and extend to almost all organs of the body and appear to increase the susceptibility of gastric mucosa to yeast cell proliferation.
本研究的目的是调查未经治疗的糖尿病对家兔组织形态学的长期并发症。通过在禁食12小时后，每周腹腔注射四剂80毫克/千克体重的四氧嘧啶，在一组新西兰雄性白兔中实验性诱导糖尿病。另一组兔子作为健康对照，以类似方式接受等渗盐水。通过空腹血糖水平确认糖尿病的建立。为了对不同器官进行组织形态学研究，7周后处死50%的动物，其余在26周后处死。使用常规苏木精和伊红染色以及Gomori改良染色。在整个实验期间，糖尿病家兔的血糖水平显著升高。血糖峰值出现在研究的第六周。此外，在糖尿病家兔的胰腺、肾脏、肺、心脏和大脑中记录到组织形态学改变。然而，在胃肠道观察到轻度变化，胃中有酵母增殖。随着未经治疗的糖尿病的进展，组织解剖学改变加剧并扩展到身体几乎所有器官，似乎增加了胃黏膜对酵母细胞增殖的易感性。
