Gok Beril, Sciubba Daniel M, Okutan Ozerk, Beskonakli Etem, Palaoglu Selcuk, Erdamar Husamettin, Sargon Mustafa F
Ankara Ataturk Research and Education Hospital, Department of Neurological Surgery, Ankara, Turkey.
J Clin Neurosci. 2009 Apr;16(4):549-53. doi: 10.1016/j.jocn.2008.04.024. Epub 2009 Feb 5.
Immunomodulation of acute spinal cord injury may inhibit the activity of specific inflammatory cascades and result in recovery of motor function. In this study, evaluation of the protective effect of a well-known anti-inflammatory immunomodulator, immunoglobulin G (IgG), was conducted in rats after a 50 g/cm contusion spinal cord injury. Following injury, 400 mg/kg of IgG was administered to the treatment group. Twenty-four hours later, animals were assessed functionally via an inclined plane and the Basso-Beattie-Bresnahan motor scale and compared to controls. Tissue was reviewed for myeloperoxidase activiy (MPO) and lipid peroxidation (LPO), and electron microscopy was conducted to assess tissue ultrastructure. Significant functional preservation was observed in the IgG treatment group. In addition, biochemical assays revealed decreased MPO activity, and electron microscopic views of tissue showed preserved ultrastructure. IgG treatment following acute contusion injury to the rat spinal cord confers functional and structural neuroprotection.
急性脊髓损伤的免疫调节可能会抑制特定炎症级联反应的活性，并导致运动功能恢复。在本研究中，对一种知名的抗炎免疫调节剂免疫球蛋白G（IgG）在大鼠50 g/cm脊髓挫伤损伤后的保护作用进行了评估。损伤后，给治疗组大鼠注射400 mg/kg的IgG。24小时后，通过斜面和Basso-Beattie-Bresnahan运动量表对动物进行功能评估，并与对照组进行比较。对组织进行髓过氧化物酶活性（MPO）和脂质过氧化（LPO）检测，并进行电子显微镜检查以评估组织超微结构。在IgG治疗组中观察到显著的功能保留。此外，生化分析显示MPO活性降低，组织电子显微镜观察显示超微结构保存。大鼠脊髓急性挫伤损伤后给予IgG治疗可提供功能和结构上的神经保护。
