Kennedy Richard B, Ovsyannikova Inna G, Pankratz V Shane, Vierkant Robert A, Jacobson Robert M, Ryan Margaret A K, Poland Gregory A
Mayo Vaccine Research Group, Mayo Clinic, Rochester, MN, USA.
Vaccine. 2009 May 26;27(25-26):3319-23. doi: 10.1016/j.vaccine.2009.01.086. Epub 2009 Feb 5.
There are no data currently available on gender and racial variation in smallpox vaccine immune responses. We recruited 1076 healthy adults 18-40 years old who received one dose of the US-licensed smallpox vaccine (Dryvax). Vaccinia neutralizing antibody titers in each subject's serum were determined using a high throughput neutralization assay based on a recombinant, beta-gal expressing vaccinia virus. Results are reported as the serum dilution inhibiting 50% of virus activity (ID(50)). The median ID(50) for all subjects was 132.2 (inter-quartile range (IQR)=78.8, 205.6). While no significant differences were observed with race and ethnicity, females had significantly higher neutralizing antibody titers than males (158.5 [93.2, 255.8] vs. 124.1 [75.2, 185.9]; p<0.0001). As expected, time since vaccination was also associated with variations in neutralizing antibody titers in our subjects. These data indicate that neutralizing antibody titers following primary smallpox vaccination vary by gender.
目前尚无关于天花疫苗免疫反应中性别和种族差异的数据。我们招募了1076名18至40岁的健康成年人，他们接种了一剂美国许可的天花疫苗（Dryvax）。使用基于表达β-半乳糖苷酶的重组痘苗病毒的高通量中和试验，测定每个受试者血清中的痘苗中和抗体滴度。结果报告为抑制50%病毒活性的血清稀释度（ID(50)）。所有受试者的ID(50)中位数为132.2（四分位间距（IQR）=78.8, 205.6）。虽然在种族和民族方面未观察到显著差异，但女性的中和抗体滴度显著高于男性（158.5 [93.2, 255.8] 对 124.1 [75.2, 185.9]；p<0.0001）。正如预期的那样，接种疫苗后的时间也与我们受试者中和抗体滴度的变化有关。这些数据表明，初次接种天花疫苗后的中和抗体滴度因性别而异。
