Emir Hatice, Kaptan Zeynep Kizilkaya, Samim Erdal, Sungu Nuran, Ceylan Kursat, Ustun Huseyin
Otorhinolaryngology Clinic, Ministry of Health, Ankara Training and Research Hospital, Turkey.
Otolaryngol Head Neck Surg. 2009 Feb;140(2):171-6. doi: 10.1016/j.otohns.2008.10.027.
To determine the preventive effect of Ginkgo biloba extract in the formation of myringosclerosis.
Prospective, randomized clinical study.
Posterosuperior quadrant of tympanic membranes of twenty-five Wistar Albino rats were bilaterally myringotomized. They were divided into three groups randomly. Groups 1 and 2 were administered 100 mg/kg/day and 200 mg/kg/day systemic Ginkgo biloba extract. The third group was treated with 1 mL/day saline solution. After 10 days of treatment, myringotomized membranes were examined by otomicroscopy and harvested. They were evaluated histopathologically by light microscopy and compared according to the occurrence of myringosclerotic plaques and the thickness of membranes.
Tympanic membranes of groups 1 and 2 nearly had no existence of myringosclerosis (72.2% in group 1 and 83.3% in group 2, P = 0.434). However, in group 3, only 14.3 percent had no myringosclerosis (P < 0.001). Thickness of membranes in group 1 was 0.13 +/- 0.25, group 2 was 0.06 +/- 0.02, and group 3 was 0.19 +/- 0.35 mm. Group 3 had significantly thicker membranes compared with groups 1 and 2 (P < 0.001 for group 2 and P = 0.003 for group 1).
Formation of experimental myringosclerosis was reduced or inhibited and tympanic membranes were thinner after systemic Ginkgo biloba extract administration.
确定银杏叶提取物对鼓膜硬化形成的预防作用。
前瞻性、随机临床研究。
对25只Wistar白化大鼠的鼓膜后上象限进行双侧鼓膜切开术。将它们随机分为三组。第1组和第2组分别给予100mg/kg/天和200mg/kg/天的全身性银杏叶提取物。第三组每天用1mL生理盐水治疗。治疗10天后，通过耳显微镜检查鼓膜切开的鼓膜并进行采集。通过光学显微镜对其进行组织病理学评估，并根据鼓膜硬化斑块的出现情况和鼓膜厚度进行比较。
第1组和第2组的鼓膜几乎不存在鼓膜硬化（第1组为72.2%，第2组为83.3%，P = 0.434）。然而，在第3组中，只有14.3%没有鼓膜硬化（P < 0.001）。第1组鼓膜厚度为0.13±0.25，第2组为0.06±0.02，第3组为0.19±0.35mm。与第1组和第2组相比，第3组的鼓膜明显更厚（第2组P < 0.001，第1组P = 0.003）。
全身性给予银杏叶提取物后，实验性鼓膜硬化的形成减少或受到抑制，且鼓膜变薄。
