Ministry of Health, Ankara Training and Research Hospital, 2nd ENT Clinic, 06340, Cebeci, Ankara, Turkey.
Eur Arch Otorhinolaryngol. 2012 Nov;269(11):2335-41. doi: 10.1007/s00405-011-1889-z. Epub 2011 Dec 25.
An antibacterial drug, ciprofloxacin, has been reported to modulate the inflammatory and immune responses on monocytes. Our objective is to evaluate the effectiveness of ciprofloxacin in the prevention of myringosclerosis that is created by myringotomy in rats and to compare it with prednisolone. In this study, 24 healthy Sprague-Dawley type rats of age 3-4 months and 250-300 g weight were used. Rats have been divided into three random groups. Topical ciprofloxacin was administered to the rats in the first group and topical prednisolone was administered to the rats in the third group; no medication was applied to their opposite ears and they were determined as control group. Prednisolone was administered to one ear of the rats and ciprofloxacin was administered to their opposite sides in the second group. After bilateral myringotomies the treatments were applied for 20 days as five drops two times a day. Otomicroscopic examination was made on the 10th day and reperforations were made when necessary. Rats were killed on the 21st day and temporal bone dissections were done. When we evaluate in terms of myringosclerosis and tympanic membrane thickness, in the first group, myringosclerosis and thickness have been observed in two of seven ears (28.6%) where ciprofloxacin was administered and in five of the seven ears (71.4%) where no treatment was applied. In the second group where prednisolone and ciprofloxacin were compared, myringosclerosis and thickness have been observed in six of the ten ears (60.0%) where prednisolone was administered and in four of the ten ears (40.0%) where ciprofloxacin was administered. In the third group, myringosclerosis and thickness have been observed in three of seven ears (42.9%) where prednisolone was administered and in four of the seven ears (57.1%) where no treatment was applied. All histopathological evaluations were made by one pathologist in a blinded manner. In our study, the effect of the ciprofloxacin and prednisolone was similar in preventing the experimental myringosclerosis and TM thickness in rats. When compared with the control groups, this preventive effect was more obvious in the ciprofloxacin treatment group than that of prednisolone.
已有报道称，一种抗菌药物环丙沙星可调节单核细胞的炎症和免疫反应。我们的目的是评估环丙沙星在预防鼓膜切开术后大鼠鼓膜硬化症中的有效性，并将其与泼尼松龙进行比较。在这项研究中，使用了 24 只年龄为 3-4 个月、体重为 250-300 克的健康 Sprague-Dawley 型大鼠。大鼠被随机分为三组。第一组大鼠给予局部环丙沙星，第三组大鼠给予局部泼尼松龙；对其对侧耳朵不给予任何药物，作为对照组。第二组大鼠一侧耳朵给予泼尼松龙，对侧耳朵给予环丙沙星。双侧鼓膜切开术后，每天两次滴 5 滴，共 20 天。第 10 天进行耳镜检查，必要时进行再穿孔。第 21 天处死大鼠，进行颞骨解剖。从鼓膜硬化和鼓膜厚度方面进行评价，在给予环丙沙星的 7 只耳朵中，有 2 只（28.6%）出现了鼓膜硬化和增厚，而在未给予任何治疗的 7 只耳朵中，有 5 只（71.4%）出现了这种情况。在比较泼尼松龙和环丙沙星的第二组中，在给予泼尼松龙的 10 只耳朵中，有 6 只（60.0%）出现了鼓膜硬化和增厚，而在给予环丙沙星的 10 只耳朵中，有 4 只（40.0%）出现了这种情况。在给予泼尼松龙的 7 只耳朵中，有 3 只（42.9%）出现了鼓膜硬化和增厚，而在未给予任何治疗的 7 只耳朵中，有 4 只（57.1%）出现了这种情况。所有的组织病理学评价均由一位病理学家在盲法下进行。在我们的研究中，环丙沙星和泼尼松龙在预防大鼠实验性鼓膜硬化和 TM 厚度方面的效果相似。与对照组相比，环丙沙星治疗组的这种预防效果比泼尼松龙更为明显。
