GC/MS 分析、抗氧化活性及（牻牛儿苗科）的抗菌作用。

GC/MS Analysis, Antioxidant Activity, and Antimicrobial Effect of (Geraniaceae).

机构信息

Doctorado en Ciencias en Procesos Biotecnológicos, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44340, Jalisco, Mexico.

Centro Universitario de Ciencias Exactas e Ingenierías, Laboratorio de Bioquímica Avanzada, Departamento de Química, Universidad de Guadalajara, Blvd. Marcelino García Barragán #1421, Guadalajara CP 44430, Jalisco, Mexico.

出版信息

Molecules. 2022 May 26;27(11):3436. doi: 10.3390/molecules27113436.

DOI:10.3390/molecules27113436

PMID:35684374

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9181846/

Abstract

In recent years, the increase in antibiotic resistance demands searching for new compounds with antimicrobial activity. Phytochemicals found in plants offer an alternative to this problem. The genus Pelargonium contains several species; some have commercial use in traditional medicine such as , and others such as are little studied but have promising potential for various applications such as phytopharmaceuticals. In this work, we characterized the freeze-dried extracts (FDEs) of five tissues (root, stem, leaf, and two types of flowers) and the ethyl acetate fractions from leaf (Lf-EtOAc) and flower (Fwr-EtOAc) of through the analysis by thin-layer chromatography (T.L.C.), gas chromatography coupled to mass spectrometry (GC-MS), phytochemicals quantification, antioxidant capacity, and antimicrobial activity. After the first round of analysis, it was observed that the FDE-Leaf and FDE-Flower showed higher antioxidant and antimicrobial activities compared to the other FDEs, for which FDE-Leaf and FDE-Flower were fractionated and analyzed in a second round. The antioxidant activity determined by ABTS showed that Lf-EtOAc and Fwr-EtOAc had the lowest IC values with 27.15 ± 1.04 and 28.11 ± 1.3 µg/mL, respectively. The content of total polyphenols was 264.57 ± 7.73 for Lf-EtOAc and 105.39 ± 4.04 mg G.A./g FDE for Fwr-EtOAc. Regarding the content of flavonoid, Lf-EtOAc and Fw-EtOAc had the highest concentration with 34.4 ± 1.06 and 29.45 ± 1.09 mg Q.E./g FDE. In addition, the minimum inhibitory concentration (M.I.C.) of antimicrobial activity was evaluated: Lf-EtOAc and Fwr-EtOAc were effective at 31.2 µg/mL for and 62.5 µg/mL for , while for the strain, Fwr-EtOAc presented 31.2 µg/mL of M.I.C. According to the GC-MS analysis, the main compounds were 1,2,3-Benzenetriol (Pyrogallol), with 77.38% of relative abundance in the Lf-EtOAc and 71.24% in the Fwr-EtOAc, followed by ethyl gallate (13.10%) in the Fwr-EtOAc and (Z)-9-Octadecenamide (13.63% and 6.75%) in both Lf-EtOAc and Fwr-EtOAc, respectively.

摘要

近年来，抗生素耐药性的增加要求寻找具有抗菌活性的新化合物。植物中的植物化学物质为解决这个问题提供了一种替代方法。天竺葵属包含几个物种；有些在传统医学中有商业用途，如，而其他如则研究较少，但在植物药等各种应用中具有很大的潜力。在这项工作中，我们通过薄层层析（TLC）、气相色谱-质谱联用（GC-MS）、植物化学物质定量分析、抗氧化能力和抗菌活性分析，对五种组织（根、茎、叶和两种类型的花）的冻干提取物（FDE）和叶（Lf-EtOAc）和花（Fwr-EtOAc）的乙酸乙酯部分进行了表征。经过第一轮分析，我们观察到与其他 FDE 相比，叶 FDE 和花 FDE 具有更高的抗氧化和抗菌活性，因此对叶 FDE 和花 FDE 进行了第二轮分离分析。ABTS 法测定的抗氧化活性表明，Lf-EtOAc 和 Fwr-EtOAc 的 IC 值最低，分别为 27.15 ± 1.04 和 28.11 ± 1.3 µg/mL。Lf-EtOAc 的总多酚含量为 264.57 ± 7.73，Fwr-EtOAc 的总多酚含量为 105.39 ± 4.04 mg G.A./g FDE。关于类黄酮的含量，Lf-EtOAc 和 Fw-EtOAc 的浓度最高，分别为 34.4 ± 1.06 和 29.45 ± 1.09 mg Q.E./g FDE。此外，还评估了最低抑菌浓度（MIC）的抗菌活性：Lf-EtOAc 和 Fwr-EtOAc 对有效，浓度为 31.2 µg/mL，对有效，浓度为 62.5 µg/mL，而对于菌株，Fwr-EtOAc 的 MIC 为 31.2 µg/mL。根据 GC-MS 分析，主要化合物为 1,2,3-苯三酚（焦倍酚），在 Lf-EtOAc 中的相对丰度为 77.38%，在 Fwr-EtOAc 中的相对丰度为 71.24%，其次是 Fwr-EtOAc 中的没食子酸乙酯（13.10%）和 Lf-EtOAc 和 Fwr-EtOAc 中的（Z）-9-十八烯酰胺（13.63%和 6.75%）。