Velásquez Lina, Dussan Jenny
Centro de investigaciones microbiológicas, Universidad de los Andes, Cra 1 N. 18 A-10, Colombia.
J Hazard Mater. 2009 Aug 15;167(1-3):713-6. doi: 10.1016/j.jhazmat.2009.01.044. Epub 2009 Jan 20.
The tolerance to As, Hg, Co, Fe and Cr was determined in different Colombian Bacillus sphaericus native strains, as well as the biosorption and bioaccumulation in living biomass. In addition, biosorption of Cr in dead cells was also determined. Living cells of the two most tolerant strains had the capacity to accumulate between 6 and 47% of Co, Hg, Fe and As. Living and dead cells of B. sphaericus OT4b31 showed a biosorption of 25 and 44.5% of Cr respectively, while B. sphaericus IV(4)10 showed a biosorption of 32 and 45%. These results are due to the absence of an active metabolism in dead cells and to the pH adjustment. S-layer proteins may possibly have the ability to entrap metallic ions, either on living or dead cells. This can be an interesting alternative for bioremediation processes of heavy metals.
测定了不同的哥伦比亚球形芽孢杆菌本地菌株对砷、汞、钴、铁和铬的耐受性，以及活生物质中的生物吸附和生物累积。此外，还测定了死细胞中铬的生物吸附。两种耐受性最强的菌株的活细胞能够累积6%至47%的钴、汞、铁和砷。球形芽孢杆菌OT4b31的活细胞和死细胞对铬的生物吸附率分别为25%和44.5%，而球形芽孢杆菌IV(4)10的生物吸附率为32%和45%。这些结果是由于死细胞中缺乏活跃的代谢以及pH调节所致。S层蛋白可能具有在活细胞或死细胞上捕获金属离子的能力。这对于重金属的生物修复过程可能是一个有趣的选择。
