Shah Abdul Qadir, Kazi Tasneem Gul, Arain Mohammad Balal, Baig Jameel Ahmed, Afridi Hassan Imran, Kandhro Ghulam Abbas, Khan Sumaira, Jamali Mohammad Khan
National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro, Pakistan.
J Hazard Mater. 2009 Aug 15;167(1-3):511-5. doi: 10.1016/j.jhazmat.2009.01.031. Epub 2009 Jan 17.
The purpose of this paper is to develop a database of fish tissues and to evaluate concentration of arsenic (As) in five tissues of fish species collected from Manchar Lake Pakistan and to compare concentration of As in fish tissues of same fish species collected from the Indus River, Pakistan. A sensitive and precise, hydride generation atomic absorption spectrometry (HG AAS) method is presented for the determination of total Arsenic (As). Microwave acid-assisted digestion (MAD) procedure based on the mixture HNO(3)/H(2)O(2) was evaluated. The method was successfully validated against CRM DORM-2 (dogfish muscle). Quantitative As recovery in CRM (DORM-2) was obtained and no statistical differences were found at 95% level by applying the t-test. The limit of detection (LOD) and limit of quantitation (LOQ), for As were established as 0.022 and 0.063 microg g(-1), respectively. The results of this study indicated that As concentration in fish tissues from the Indus River are generally lower than in tissues of fishes from Manchar Lake. Arsenic concentrations in fish tissues of Indus River are although above the respective human health-based concentrations.
本文的目的是建立一个鱼类组织数据库，评估从巴基斯坦曼查尔湖采集的鱼类物种的五种组织中砷（As）的浓度，并比较从巴基斯坦印度河采集的同一种鱼类的鱼类组织中As的浓度。提出了一种灵敏、精确的氢化物发生原子吸收光谱法（HG AAS）用于测定总砷（As）。评估了基于HNO₃/H₂O₂混合物的微波酸辅助消解（MAD）程序。该方法成功地针对CRM DORM-2（鲨鱼肌肉）进行了验证。获得了CRM（DORM-2）中As的定量回收率，通过应用t检验在95%水平上未发现统计学差异。As的检测限（LOD）和定量限（LOQ）分别确定为0.022和0.063μg g⁻¹。本研究结果表明，印度河鱼类组织中的As浓度通常低于曼查尔湖鱼类组织中的浓度。印度河鱼类组织中的砷浓度虽然高于各自基于人类健康的浓度。
