Tsai Chia-Fang, Hsu Yu-Wen, Chen Wen-Kang, Ho Yung-Chyuan, Lu Fung-Jou
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan.
Biosci Biotechnol Biochem. 2009 Feb;73(2):280-7. doi: 10.1271/bbb.80413. Epub 2009 Feb 7.
Electrolzyed-reduced water (ERW) is a higher pH and lower oxidation-reduction potential water. In the present study, we examined the enhanced effect of ERW in the apoptosis of leukemia cells (HL-60) induced by glutathione (GSH). An enhanced inhibitory effect on the viability of the HL-60 cells was observed after treatment with a combination of ERW with various concentrations of GSH, whereas no cytotoxic effect in normal peripheral blood mononuclear cells was observed. The results of apoptotic related protein indicated that the induction of HL-60 cell death was caused by the induction of apoptosis through upregulation of Bax and downregulation of Bcl-2. The results of further investigation showed a diminution of intracellular GSH levels in ERW, and combination with GSH groups. These results suggest that ERW is an antioxidant, and that ERW, in combination with GSH, has an enhanced apoptosis-inducing effect on HL-60 cells, which might be mediated through the mitochondria-dependent pathway.
电解还原水（ERW）是一种具有较高pH值和较低氧化还原电位的水。在本研究中，我们检测了电解还原水对谷胱甘肽（GSH）诱导白血病细胞（HL-60）凋亡的增强作用。用不同浓度的谷胱甘肽与电解还原水联合处理后，观察到对HL-60细胞活力有增强的抑制作用，而在正常外周血单个核细胞中未观察到细胞毒性作用。凋亡相关蛋白的结果表明，HL-60细胞死亡的诱导是通过上调Bax和下调Bcl-2诱导凋亡所致。进一步研究结果显示，电解还原水组以及电解还原水与谷胱甘肽联合组细胞内谷胱甘肽水平降低。这些结果表明，电解还原水是一种抗氧化剂，并且电解还原水与谷胱甘肽联合对HL-60细胞具有增强的凋亡诱导作用，这可能是通过线粒体依赖途径介导的。
