Sakano Noriko, Takahashi Noriko, Wang Da-Hong, Sauriasari Rani, Takemoto Kei, Kanbara Sakiko, Sato Yoshie, Takigawa Tomoko, Takaki Jiro, Ogino Keiki
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Japan.
Free Radic Res. 2009 Feb;43(2):183-92. doi: 10.1080/10715760802663124.
To examine the relation between lifestyle factors and oxidative stress biomarkers, this study analysed plasma 3-nitrotyrosine (3-NT), urinary 8-isoprostane and 8-hydroxy-2'- deoxyguanosine (8-OHdG) of 323 healthy Japanese without any disease. Plasma 3-NT was significantly increased by excessive exercise (p=0.010), but it was not significantly different in terms of sex, age (< 40, >==40), BMI (<18.5, 18.5-24.9, >==25.0), smoking (non-smokers, smokers) and alcohol drinking per week (non-drinkers, <10 units, >==10 units). Urinary 8-isoprostane was significantly associated with alcohol drinking (p <0.01) and sex (p <0.01), although it had no significant relevance to age and exercise. Moreover, urinary 8-OHdG was positively associated with age (p <0.05) and negatively associated with BMI (p <0.05) and fasting insulin (p <0.001). However, it was not related with sex, smoking, alcohol drinking and exercise. In conclusion, the present results suggest that 3-NT, 8-isoprostane and 8-OHdG seem to be useful biomarkers for early prediction of lifestyle-related disease risk at the population level.
为了研究生活方式因素与氧化应激生物标志物之间的关系，本研究分析了323名无任何疾病的健康日本受试者的血浆3-硝基酪氨酸（3-NT）、尿8-异前列腺素和8-羟基-2'-脱氧鸟苷（8-OHdG）。过度运动使血浆3-NT显著升高（p = 0.010），但在性别、年龄（<40岁、≥40岁）、体重指数（<18.5、18.5 - 24.9、≥25.0）、吸烟（非吸烟者、吸烟者）以及每周饮酒量（不饮酒者、<10单位、≥10单位）方面无显著差异。尿8-异前列腺素与饮酒（p <0.01）和性别（p <0.01）显著相关，尽管与年龄和运动无显著相关性。此外，尿8-OHdG与年龄呈正相关（p <0.05），与体重指数呈负相关（p <0.05），与空腹胰岛素呈负相关（p <0.001）。然而，它与性别、吸烟、饮酒和运动无关。总之，目前的结果表明，3-NT、8-异前列腺素和8-OHdG似乎是在人群水平上早期预测生活方式相关疾病风险的有用生物标志物。
