日本工人的闲暇时间体力活动与 DNA 损伤。

Leisure-time physical activity and DNA damage among Japanese workers.

机构信息

Faculty of Sport Sciences, Waseda University, Tokorozawa, Japan.

Department of Epidemiology and Prevention, Center for Clinical Sciences, National Center for Global Health and Medicine, Tokyo, Japan.

出版信息

PLoS One. 2019 Feb 15;14(2):e0212499. doi: 10.1371/journal.pone.0212499. eCollection 2019.

DOI:10.1371/journal.pone.0212499

PMID:30768632

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6377137/

Abstract

BACKGROUND

It remains unclear whether daily physical activity is associated with DNA damage. This cross-sectional study examined the association between leisure-time physical activity and urinary 8-hydroxydeoxyguanosine (8-OH-dG), a biomarker of oxidative DNA damage, or urinary 7-methylguanine (m7Gua), a biomarker of methylating DNA damage.

METHODS

Participants included 501 workers (294 men and 207 women), aged 20-65 years, from municipal offices in Japan. Urinary 8-OH-dG and m7Gua were measured using column-switching HPLC. Physical activity was evaluated using a self-reported questionnaire. The associations between leisure-time physical activity and urinary DNA damage markers were assessed by multiple linear regression analysis, with stratification by occupational physical activity.

RESULTS

After adjusting for covariates, leisure-time physical activity showed a suggestive inverse correlation with urinary 8-OH-dG levels (P for trend = 0.06), and a significant inverse association with urinary m7Gua levels (P for trend = 0.03). In analysis stratified by occupation, inverse correlations were observed in sedentary workers (walking < 30 min/day at work: P for trend = 0.06 and = 0.03 for urinary 8-OH-dG and m7Gua, respectively), but not in physically active workers (walking ≥ 30 min/day at work). In analysis for each intensity of leisure-time physical activity, light-intensity exercise was associated with lower levels of urinary 8-OH-dG (P for trend = 0.03), whereas moderate-to-high-intensity exercise was associated with lower levels of urinary m7Gua (P for trend = 0.02).

CONCLUSIONS

Our results suggest that high levels of leisure-time physical activity are associated with decreased levels of DNA damage in individuals with low physical activity at work.

摘要

背景

目前尚不清楚日常体力活动是否与 DNA 损伤有关。本横断面研究调查了休闲时间体力活动与尿 8-羟基脱氧鸟苷（8-OH-dG）——一种氧化 DNA 损伤的生物标志物，或尿 7-甲基鸟嘌呤（m7Gua）——一种甲基化 DNA 损伤的生物标志物之间的关系。

方法

参与者包括来自日本市政办公室的 501 名工人（294 名男性和 207 名女性），年龄在 20-65 岁之间。使用柱切换 HPLC 测量尿 8-OH-dG 和 m7Gua。使用自我报告问卷评估体力活动。采用多元线性回归分析，按职业体力活动分层，评估休闲时间体力活动与尿 DNA 损伤标志物之间的关系。

结果

调整协变量后，休闲时间体力活动与尿 8-OH-dG 水平呈明显负相关（趋势 P = 0.06），与尿 m7Gua 水平呈显著负相关（趋势 P = 0.03）。按职业分层分析，在久坐不动的工人中观察到负相关（工作时步行<30 分钟/天：尿 8-OH-dG 和 m7Gua 的趋势 P 值分别为 0.06 和 0.03），但在体力活动较多的工人中没有观察到负相关（工作时步行≥30 分钟/天）。在对每种强度的休闲时间体力活动进行分析时，轻度运动与较低水平的尿 8-OH-dG 相关（趋势 P = 0.03），而中高强度运动与较低水平的尿 m7Gua 相关（趋势 P = 0.02）。