Wu Changfeng, Bull Barbara, Szymanski Craig, Christensen Kenneth, McNeill Jason
Department of Chemistry, Clemson University, Clemson, South Carolina 29634, USA.
ACS Nano. 2008 Nov 25;2(11):2415-23. doi: 10.1021/nn800590n.
Highly fluorescent conjugated polymer dots were developed for demanding applications such as fluorescence imaging in live cells. These nanoparticles exhibit small particle diameters, extraordinary fluorescence brightness, and excellent photostability. Single particle fluorescence imaging and kinetic studies indicate much higher emission rates (approximately 10(8) s(-1)) and little or no blinking of the nanoparticles as compared to typical results for single dye molecules and quantum dots. Analysis of single particle photobleaching trajectories reveals excellent photostabilityas many as 10(9) or more photons emitted per nanoparticle prior to irreversible photobleaching. The superior figures of merit of these new fluorescent probes, together with the demonstration of cellular imaging, indicate their enormous potential for demanding fluorescence-based imaging and sensing applications such as high speed super-resolution single molecule/particle tracking and highly sensitive assays.
高荧光共轭聚合物点被开发用于诸如活细胞荧光成像等苛刻应用。这些纳米粒子具有小粒径、非凡的荧光亮度和出色的光稳定性。单粒子荧光成像和动力学研究表明，与单染料分子和量子点的典型结果相比，这些纳米粒子的发射速率要高得多（约10⁸ s⁻¹），并且几乎没有或根本没有闪烁现象。对单粒子光漂白轨迹的分析揭示了其出色的光稳定性——在不可逆光漂白之前，每个纳米粒子可发射多达10⁹个或更多的光子。这些新型荧光探针优异的品质因数，连同细胞成像的证明，表明它们在诸如高速超分辨率单分子/粒子跟踪和高灵敏度检测等苛刻的基于荧光的成像和传感应用中具有巨大潜力。
