Australian Institute of Marine Science, Australia.
J Nat Prod. 2009 Feb 27;72(2):290-4. doi: 10.1021/np800562m.
Dereplication of a methanolic extract of the marine sponge Ianthella flabelliformis using FTICR-MS accurate mass determination and MS(n) techniques enabled rapid and unambiguous detection of a new compound among a plethora of known compounds. Isolation of this compound and the known 19-deoxy analogue using the hyphenated technique LC-UV/MS-SPE-NMR was undertaken, and the structures were confirmed, from a single chromatographic run, as 19-hydroxyaraplysillin-I N(20)-sulfamate (1) and araplysillin-I N(20)-sulfamate (2).
利用傅立叶变换离子回旋共振质谱（FTICR-MS）精确质量测定和 MS(n) 技术对海洋海绵 Ianthella flabelliformis 的甲醇提取物进行去重复化处理，可在众多已知化合物中快速、明确地检测到一种新化合物。采用 LC-UV/MS-SPE-NMR 偶联技术对该化合物和已知的 19-去氧类似物进行分离，从单次色谱运行中即可确定其结构为 19-羟基阿普里西林-I N(20)-磺胺（1）和阿普里西林-I N(20)-磺胺（2）。
