Wang Li, Li Yubao, Zuo Yi, Zhang Li, Zou Qin, Cheng Lin, Jiang Hong
Research Center for Nano-Biomaterials, Analytical and Testing Center, Sichuan University, Chengdu 610064, People's Republic of China.
Biomed Mater. 2009 Apr;4(2):025003. doi: 10.1088/1748-6041/4/2/025003. Epub 2009 Feb 11.
In this study, a new hydroxyapatite (HA)/polyurethane (PU) composite porous scaffold was developed by in situ polymerization. Aliphatic isophorone diisocyanate as a nontoxic and safe agent was adopted to produce the rigid segment in polyurethane polymerization. Hydroxyapatite powder was compounded in a PU polymer matrix during the polymeric process. The macrostructure and morphology as well as mechanical strength of the scaffolds were characterized by FTIR, XRD, DSC and SEM. The results show that the isophorone diisocyanate can react mildly with hydroxyl (-OH) groups of castor oil and a mild foaming action caused by the release of CO2 gas occurred simultaneously in the reactive process, thus producing a uniform porous structure of HA/PU scaffold. The HA/PU composite scaffold with a high HA content of about 60 wt% has a porosity of more than 78% and a pore size from 100 microm to 800 microm. The HA/PU scaffold exhibited good cytocompatibility estimated by co-culturing the scaffold with MG63 cells through MTT test. The porous composite scaffold has good homogenization and a perfect three-dimensional structure for cell migration and bone tissue ingrowth, and should have good prospects for bone tissue regeneration.
在本研究中，通过原位聚合法制备了一种新型羟基磷灰石（HA）/聚氨酯（PU）复合多孔支架。采用无毒安全的脂肪族异佛尔酮二异氰酸酯在聚氨酯聚合过程中生成刚性链段。在聚合过程中，将羟基磷灰石粉末与PU聚合物基体复合。通过傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、差示扫描量热法（DSC）和扫描电子显微镜（SEM）对支架的宏观结构、形态以及力学强度进行了表征。结果表明，异佛尔酮二异氰酸酯能与蓖麻油中的羟基（-OH）基团发生温和反应，并且在反应过程中同时发生由二氧化碳气体释放引起的温和发泡作用，从而制备出具有均匀多孔结构的HA/PU支架。HA含量约为60 wt%的HA/PU复合支架孔隙率超过78%，孔径为100微米至800微米。通过MTT试验将支架与MG63细胞共培养评估得出，HA/PU支架表现出良好的细胞相容性。该多孔复合支架具有良好的均匀性以及有利于细胞迁移和骨组织长入的完美三维结构，在骨组织再生方面应具有良好的前景。
