Vargas Gil S, Pastor S, March G J
Instituto de Fitopatología y Fisiología Vegetal, Córdoba, Argentina.
Microbiol Res. 2009;164(2):196-205. doi: 10.1016/j.micres.2006.11.022. Epub 2007 Apr 24.
Soil biodiversity plays a key role in the sustainability of agriculture systems and indicates the level of health of soil, especially when considering the richness of microorganisms that are involved in biological control of soilborne diseases. Cultural practices may produce changes in soil microflora, which can be quantified through the isolation of target microorganisms. Rhizosphere soil samples were taken from an assay with different crop rotations and tillage systems, and populations of Trichoderma spp., Gliocladium spp. and actinomycetes were quantified in order to select the general and selective culture media that better reflect the changes of these microbial populations in soil. The most efficient medium for the isolation of Trichoderma spp. and Gliocladium spp. was potato dextrose agar modified by the addition of chloramphenicol, streptomycin and rose bengal, and for actinomycetes was Küster medium, with cycloheximide and sodium propionate.
土壤生物多样性在农业系统的可持续性中起着关键作用，并指示土壤的健康水平，尤其是考虑到参与土传病害生物防治的微生物丰富度时。栽培措施可能会导致土壤微生物区系发生变化，这可以通过分离目标微生物来进行量化。从不同作物轮作和耕作系统的试验中采集根际土壤样本，并对木霉属、粘帚霉属和放线菌的数量进行量化，以选择能更好反映这些微生物种群在土壤中变化的通用和选择性培养基。分离木霉属和粘帚霉属最有效的培养基是添加了氯霉素、链霉素和孟加拉红改良的马铃薯葡萄糖琼脂，而分离放线菌最有效的培养基是添加了放线菌酮和丙酸钠的库斯特培养基。
