Schreiber Mark J, Holmes Edward C, Ong Swee Hoe, Soh Harold S H, Liu Wei, Tanner Lukas, Aw Pauline P K, Tan Hwee Cheng, Ng Lee Ching, Leo Yee Sin, Low Jenny G H, Ong Adrian, Ooi Eng Eong, Vasudevan Subhash G, Hibberd Martin L
Novartis Institute for Tropical Diseases, Chromos, Singapore.
J Virol. 2009 May;83(9):4163-73. doi: 10.1128/JVI.02445-08. Epub 2009 Feb 11.
Dengue is one of the most important emerging diseases of humans, with no preventative vaccines or antiviral cures available at present. Although one-third of the world's population live at risk of infection, little is known about the pattern and dynamics of dengue virus (DENV) within outbreak situations. By exploiting genomic data from an intensively studied major outbreak, we are able to describe the molecular epidemiology of DENV at a uniquely fine-scaled temporal and spatial resolution. Two DENV serotypes (DENV-1 and DENV-3), and multiple component genotypes, spread concurrently and with similar epidemiological and evolutionary profiles during the initial outbreak phase of a major dengue epidemic that took place in Singapore during 2005. Although DENV-1 and DENV-3 differed in viremia and clinical outcome, there was no evidence for adaptive evolution before, during, or after the outbreak, indicating that ecological or immunological rather than virological factors were the key determinants of epidemic dynamics.
登革热是人类最重要的新发疾病之一，目前尚无预防性疫苗或抗病毒治疗方法。尽管世界三分之一的人口面临感染风险，但对于登革热病毒（DENV）在疫情中的传播模式和动态却知之甚少。通过利用对一次深入研究的重大疫情的基因组数据，我们能够以独特的高精细时间和空间分辨率描述DENV的分子流行病学。在2005年新加坡发生的一次重大登革热疫情的初始爆发阶段，两种DENV血清型（DENV-1和DENV-3）以及多个组分基因型同时传播，且具有相似的流行病学和进化特征。尽管DENV-1和DENV-3在病毒血症和临床结果方面存在差异，但在疫情之前、期间或之后均没有适应性进化的证据，这表明生态或免疫因素而非病毒学因素是疫情动态的关键决定因素。
