尼帕病毒高频编辑其P基因以表达V蛋白和W蛋白。
Nipah virus edits its P gene at high frequency to express the V and W proteins.
作者信息
Kulkarni Sachin, Volchkova Valentina, Basler Christopher F, Palese Peter, Volchkov Viktor E, Shaw Megan L
机构信息
Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029, USA.
出版信息
J Virol. 2009 Apr;83(8):3982-7. doi: 10.1128/JVI.02599-08. Epub 2009 Feb 11.
Abstract
Nipah virus (NiV) is predicted to encode four proteins from its P gene (P, V, W, and C) via mRNA editing and an alternate open reading frame. By use of specific antibodies, the expression of the V, W, and C proteins in NiV-infected cells has now been confirmed. Analysis of the P-gene transcripts shows a ratio of P:V:W mRNA of 1:1:1, but this differs over time, with greater proportions of V and W transcripts observed as the infection progresses. Eighty-two percent of transcripts are edited, with up to 11 G insertions observed. This exceptionally high editing frequency ensures expression of the V and W proteins.
摘要
尼帕病毒（NiV）预计通过mRNA编辑和一个替代开放阅读框从其P基因编码四种蛋白质（P、V、W和C）。通过使用特异性抗体，现已证实V、W和C蛋白在尼帕病毒感染细胞中的表达。对P基因转录本的分析显示P:V:W mRNA的比例为1:1:1，但随着时间推移这一比例会发生变化，随着感染进展，观察到V和W转录本的比例更高。82%的转录本发生编辑，观察到多达11个G插入。这种异常高的编辑频率确保了V和W蛋白的表达。
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