通过微核试验检测漱口水对上皮细胞的细胞毒性。
Cytotoxicity of mouthrinses on epithelial cells by micronucleus test.
作者信息
Erdemir Ebru Olgun, Sengün Abdulkadir, Ulker Mustafa
机构信息
Assistant Professor, Kirikkale University, Faculty of Dentistry, Department of Periodontology, Kirikkale, Turkey.
出版信息
Eur J Dent. 2007 Apr;1(2):80-5.
OBJECTIVES
To determine the cytotoxicity of three commercial mouthrinses Klorhex, Andorex and Tanflex on buccal epithelial cells using micronucleus (MN) test.
MATERIALS AND METHODS
28 patients with aged 16-24 undergone three mouthrinses' application were analyzed before and after one week exposure. Physiologic saline was used for the control group. The MN incidence was scored in the buccal epithelial of each participants. The difference in pre- and post-treatment after one week incidence of MN and plaque (PI) and gingival indices (GI) was compared by non-parametric statistical tests.
RESULTS
The micronuclei incidence increased in Klorhex, Tanflex and Andorex groups after exposure to mouth rinses (P<.05). But when compared with the control group, there was not any difference between Andorex and control group (P>.05). In the other study groups, MN incidence was significantly increased after 7 days treatment (P<.05). GI scores of all groups were decreased significantly (P<.05). PI scores were decreased only in the Klorhex group (P<.05).
CONCLUSIONS
Our primary findings support the presence of possible cytotoxic effects of the mouthrinses on gingival epithelial cells.
目的
使用微核（MN）试验确定三种市售漱口水Klorhex、Andorex和Tanflex对颊上皮细胞的细胞毒性。
材料与方法
对28名年龄在16 - 24岁之间使用过三种漱口水的患者在暴露一周前后进行分析。生理盐水用于对照组。对每位参与者颊上皮细胞中的微核发生率进行评分。通过非参数统计检验比较一周后微核发生率、菌斑指数（PI）和牙龈指数（GI）治疗前后的差异。
结果
使用漱口水后，Klorhex、Tanflex和Andorex组的微核发生率增加（P<0.05）。但与对照组相比，Andorex组与对照组之间无差异（P>0.05）。在其他研究组中，治疗7天后微核发生率显著增加（P<0.05）。所有组的牙龈指数评分均显著降低（P<0.05）。菌斑指数评分仅在Klorhex组降低（P<0.05）。
结论
我们的主要研究结果支持漱口水可能对牙龈上皮细胞产生细胞毒性作用。
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