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三种不同市售椅旁致龋菌检测试剂盒——龋病风险检测、唾液变形链球菌检测和龋易感性检测——的效果评估

Evaluation of Efficacy of Three Different Commercially Available Kit for Chairside Cariogenic Bacteria Test - Caries Risk Test, Saliva-check Mutans and CariScreen.

作者信息

Babu Vidya, Hegde Sundeep, Bhat Sham, Sargod Sharan

机构信息

Pedodontics and Preventive Dentistry, Yenepoya Dental College, Mangalore, IND.

出版信息

Cureus. 2019 Dec 29;11(12):e6504. doi: 10.7759/cureus.6504.

Abstract

Background and objectives The presence of mutans streptococci has been used in individual assessments of caries risk. In the modern era of dentistry, the chair side kits for assessing chair side cariogenic bacteria play a significant role. There is paucity of literature about the comparison of commercially available chair side caries risk tests. Hence this study was conducted to compare the efficacy of three commercially available chair side cariogenic bacteria tests. Methodology Twenty-five patients in the age group of 5-14 years were selected. The saliva samples of patients were collected and were taken for cariogenic bacteria tests using caries risk test (CRT) bacteria test kit and saliva check mutans kit (mutans rapid detection kit). The plaque samples were taken for CariScreen caries susceptibility testing meter. All the samples were compared with a gold standard, i.e., mitis salivarius-bacitracin (MSB) agar plate test. Results Results demonstrated that the specificity of CariScreen and caries risk test was 91.67 whereas it was 75.00 for saliva check mutans. The CariScreen produced the risk status of the patient in shortest time. However, all the chair side kits failed to show the exact colony count of bacteria. Conclusion The result of the current study proved that both CariScreen and caries risk test are highly efficient in assessing the caries risk of patients. However, the CariScreen is easy to perform and provides the result in shorter time.

摘要

背景与目的 变形链球菌的存在已被用于龋齿风险的个体评估。在现代牙科时代,用于评估椅旁致龋菌的椅旁检测试剂盒发挥着重要作用。关于市售椅旁龋齿风险检测方法比较的文献较少。因此,本研究旨在比较三种市售椅旁致龋菌检测方法的有效性。方法 选取25名年龄在5至14岁之间的患者。收集患者的唾液样本,使用龋齿风险检测(CRT)细菌检测试剂盒和唾液变链菌检测试剂盒(变链菌快速检测试剂盒)对唾液样本进行致龋菌检测。采集菌斑样本用于CariScreen龋齿易感性测试仪检测。所有样本均与金标准,即唾液轻链球菌 - 杆菌肽(MSB)琼脂平板试验进行比较。结果 结果表明,CariScreen和龋齿风险检测的特异性为91.67,而唾液变链菌检测试剂盒的特异性为75.00。CariScreen在最短时间内得出患者的风险状况。然而,所有椅旁检测试剂盒均未能显示出确切的细菌菌落计数。结论 本研究结果证明,CariScreen和龋齿风险检测在评估患者龋齿风险方面均具有高效性。然而,CariScreen操作简便,能在更短时间内得出结果。

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