Pereira Treville, Aswathy J, Shetty Subraj, Tamgadge Avinash, Tamgadge Sandhya, Gotmare Swati
Department of Oral and Maxillofacial Pathology and Microbiology, School of Dentistry, D. Y. Patil University, Navi Mumbai, Maharashtra, India.
Indian Dermatol Online J. 2019 Jul-Aug;10(4):431-436. doi: 10.4103/idoj.IDOJ_147_18.
Oral lichen planus (OLP) is characterized histologically by epithelial basal cell destruction and a dense subepithelial lymphocytic infiltrate. Mast cells (MCs) play a role in the pathogenesis and progression of the disease causing changes in the basement membrane (BM). BM is seen as continuous or fragmented, distinct or indistinct, and afibrillar or fibrillar extensions.
This study was done to demonstrate the BM using acriflavine stain in addition to hematoxylin and eosin (H-E) stain. An attempt was also made to study MC using Azure A stain and assess the degree of changes in the thickness of BM associated with degranulated MC in patients with OLP.
A total of 66 paraffin-embedded tissue sections which included 30 inflamed gingival mucosa (IGM) and 36 OLP were stained with H-E stain, Azure A, and fluorescent periodic acid-acriflavine stain.
MC density was higher in OLP when compared with MC in IGM. Degranulated MCs were found in abundance in OLP. Thickness of BM was significantly less in OLP when compared with IGM. Significant fragmentation was seen in OLP when compared with BM of IGM.
Degranulated MC in OLP may or may not alter the quality of BM but definitely seems to influence the thickness of the BM both directly and indirectly.
口腔扁平苔藓（OLP）在组织学上的特征为上皮基底细胞破坏和上皮下密集的淋巴细胞浸润。肥大细胞（MCs）在该疾病的发病机制和进展中发挥作用，导致基底膜（BM）发生变化。基底膜表现为连续或断裂、清晰或模糊、无纤维或有纤维延伸。
本研究除苏木精和伊红（H-E）染色外，还使用吖啶黄素染色来显示基底膜。同时尝试使用天青A染色研究肥大细胞，并评估OLP患者中与脱颗粒肥大细胞相关的基底膜厚度变化程度。
总共66个石蜡包埋组织切片，其中包括30个炎症性牙龈黏膜（IGM）和36个OLP，用H-E染色、天青A染色和荧光高碘酸-吖啶黄素染色。
与IGM中的肥大细胞相比，OLP中的肥大细胞密度更高。在OLP中发现大量脱颗粒肥大细胞。与IGM相比，OLP中基底膜的厚度明显更薄。与IGM的基底膜相比，OLP中可见明显的断裂。
OLP中的脱颗粒肥大细胞可能会也可能不会改变基底膜的质量，但肯定似乎会直接或间接影响基底膜的厚度。
