Clinic for Preventive Dentistry, Periodontology and Cariology, University of Zurich, Plattenstr. 11, CH-8032 Zurich, Switzerland.
Clin Oral Investig. 2009 Dec;13(4):473-8. doi: 10.1007/s00784-009-0253-9. Epub 2009 Feb 13.
The aim of the present study was to analyze in how far drying of eroded dentin and enamel surfaces influence the results of profilometrical determinations with a stylus profilometer. Each five dentin and enamel samples were eroded with HCl (pH 2.6, 2 min). Surface profiles of the samples were recorded with a stylus profilometer in three series. In series 1, the samples were measured while stored in water and in series 2, under ambient conditions (21 degrees C, 35% humidity). In series 3, samples were completely desiccated and then rewetted. Profilometry was conducted at various time intervals for a period of up to 181 min (series 1 and 2) and 72 h (series 3). Only the dentin samples were affected by the storage conditions. Stable profilometrical readings for the eroded dentin samples were only feasible when the specimens were stored in water during the complete period of the experiment, including the profilometrical measurement. Thus, for erosion experiments using profilometrical analysis with a stylus profilometer, it is advised to store and measure dentin samples under wet conditions.
本研究的目的是分析在多大程度上干燥侵蚀的牙本质和牙釉质表面会影响触针轮廓仪的轮廓测定结果。每个牙本质和牙釉质样本各五个，用 HCl（pH 值 2.6，2 分钟）进行侵蚀。使用触针轮廓仪在三个系列中记录样本的表面轮廓。在系列 1 中，样本在储存在水中和在系列 2 中，在环境条件下（21°C，35%湿度）下进行测量。在系列 3 中，样本完全干燥然后重新润湿。在长达 181 分钟（系列 1 和 2）和 72 小时（系列 3）的时间间隔内进行轮廓测量。只有牙本质样本受到存储条件的影响。只有当标本在整个实验期间，包括轮廓测量，都储存在水中时，侵蚀牙本质样本才能获得稳定的轮廓测量读数。因此，对于使用触针轮廓仪进行轮廓分析的侵蚀实验，建议在湿条件下储存和测量牙本质样本。
