Wiegand A, Schneider S, Sener B, Roos M, Attin T
Department of Preventive Dentistry, Periodontology and Cariology, University of Göttingen, Göttingen, Germany.
Caries Res. 2014;48(2):154-62. doi: 10.1159/000353143. Epub 2014 Jan 3.
This study aimed to analyse the impact of brushing on the protective effect of different fluoride solutions on enamel and dentin erosion. Bovine enamel and dentin specimens were rinsed once with TiF4, AmF, SnF2 (0.5 M F, 2 min) or water (control). Specimens were either left unbrushed or brushed with 10, 20, 50, 100 or 500 brushing strokes in an automatic brushing machine (2 N, non-fluoridated toothpaste slurry). Ten specimens per group were eroded with hydrochloric acid (HCl) (pH 2.3) for 60 s, and calcium release into the acid was determined by atomic absorption spectroscopy. Additionally, enamel and dentin surfaces were analysed by X-ray energy-dispersive spectroscopy (EDS) (n = 6/group) and scanning electron microscopy (SEM) (n = 2/group) before brushing and after 500 brushing strokes. Statistical analysis (p < 0.05) was performed by three- and one-way ANOVA (calcium release) or repeated measures ANOVA (EDS). TiF4, AmF and SnF2 reduced the erosive calcium loss in unbrushed specimens to 58-67% (enamel) and 23-31% (dentin) of control. Calcium release increased with increasing brushing strokes prior to erosion and amounted to 70-88% (enamel) and 45-78% (dentin) of control after 500 brushing strokes. Brushing reduced the surface concentration of fluoride (AmF), tin (SnF2) and titanium (TiF4). SEM revealed that surface precipitates were affected by long-term brushing. Brushing reduced the protective potential of TiF4, AmF and SnF2 solutions. However, considering a small number of brushing strokes, the protective effect of fluoride solutions is only slightly affected by brushing abrasion.
本研究旨在分析刷牙对不同氟溶液对牙釉质和牙本质侵蚀的保护作用的影响。牛牙釉质和牙本质标本用四氟化钛（TiF4）、氨甲环酸氟（AmF）、氟化亚锡（SnF2）（0.5M氟，2分钟）或水（对照）冲洗一次。标本要么不刷牙，要么在自动刷牙机中用10、20、50、100或500次刷牙 strokes（2N，不含氟牙膏浆）刷牙。每组十个标本用盐酸（HCl）（pH 2.3）侵蚀60秒，通过原子吸收光谱法测定酸中钙的释放量。此外，在刷牙前和500次刷牙 strokes后，通过X射线能量色散光谱法（EDS）（每组n = 6）和扫描电子显微镜（SEM）（每组n = 2）分析牙釉质和牙本质表面。通过三因素和单因素方差分析（钙释放）或重复测量方差分析（EDS）进行统计分析（p < 0.05）。TiF4、AmF和SnF2将未刷牙标本中的侵蚀性钙损失降低至对照的58 - 67%（牙釉质）和23 - 31%（牙本质）。在侵蚀前，钙释放量随着刷牙 strokes次数的增加而增加，在500次刷牙 strokes后达到对照的70 - 88%（牙釉质）和45 - 78%（牙本质）。刷牙降低了氟（AmF）、锡（SnF2）和钛（TiF4）的表面浓度。SEM显示表面沉淀物受到长期刷牙的影响。刷牙降低了TiF4、AmF和SnF2溶液的保护潜力。然而，考虑到少量的刷牙 strokes，氟溶液的保护作用仅受到刷牙磨损的轻微影响。
