SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa.
Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa.
Int J Environ Res Public Health. 2021 Jan 8;18(2):481. doi: 10.3390/ijerph18020481.
The prevalence of bacteria with multidrug-resistance (MDR) is a significant threat to public health globally. spp. are naturally ubiquitous, with particularly being ranked as important foodborne disease-causing microorganisms. This study aimed to evaluate the incidence and determine the antimicrobial resistance (AMR) profiles of multidrug-resistant spp. (MDRL) isolated from different environmental samples (river and irrigation water) in the Sarah Baartman District Municipality (SBDM), Eastern Cape Province (ECP), South Africa. Molecular identification and characterization were carried out using polymerase chain reaction (PCR) and isolates that exhibited phenotypic resistance were further screened for relevant antimicrobial-resistant genes (ARGs). Findings revealed a total of 124 presumptive isolates; 69 were molecularly confirmed species. Out of the confirmed species, 41 isolates (59%) were classified as while 9 (13%) were classified as . All spp. exhibited phenotypic resistance against ampicillin, penicillin, and trimethoprim-sulphamethoxazole and further screening revealed ARGs in the following proportions: (71%), (66%), (63%), and (33%). Results confirmed the occurrence of ARGs among inhabiting surface waters of ECP. The present study indicates that the river water samples collected from SBDM are highly contaminated with MDRL, hence, constituting a potential health risk.
耐多药细菌（MDR）的流行是对全球公共卫生的重大威胁。 spp. 广泛存在于自然界中，其中 被特别列为重要的食源性病原体。本研究旨在评估南非东开普省萨拉·巴特曼地区都市（SBDM）不同环境样本（河流和灌溉水）中分离的耐多药 spp.（MDRL）的发生率，并确定其抗生素耐药性（AMR）谱。采用聚合酶链反应（PCR）进行分子鉴定和特征分析，对表现出表型耐药的分离株进一步筛选相关的抗生素耐药基因（ARGs）。结果共发现 124 株疑似 分离株；其中 69 株经分子确认为 种。在确认的种中，41 株（59%）被归类为 ，9 株（13%）被归类为 。所有 spp. 对氨苄西林、青霉素和甲氧苄啶-磺胺甲恶唑表现出表型耐药，进一步筛选发现以下比例的 ARGs： （71%）、 （66%）、 （63%）和 （33%）。结果证实了 ARGs 在 ECP 地表水栖息的 中存在。本研究表明，从 SBDM 采集的河水样本高度污染了 MDRL，因此构成了潜在的健康风险。
