Mota María Inés, Vázquez Sylvia, Cornejo Cecilia, D'Alessandro Bruno, Braga Valeria, Caetano Ana, Betancor Laura, Varela Gustavo
Departamento de Bacteriología y Virología, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Montevideo, Uruguay.
Departamento de Desarrollo Biotecnológico, Facultad de Medicina, Instituto de Higiene, Universidad de la República, Montevideo, Uruguay.
Front Vet Sci. 2020 Nov 6;7:583930. doi: 10.3389/fvets.2020.583930. eCollection 2020.
Shiga toxin-producing (STEC) and are worldwide recognized zoonotic pathogens. Recent reports have emerged about the circulation of antimicrobial-resistant STEC and isolates. To assess the frequency of antimicrobial resistance and related genes in these pathogens, we studied 45 STEC and 50 isolates locally recovered from different sources. Antimicrobial susceptibility testing was performed by disk-diffusion method, and the genomic sequences of three selected STEC and from all 50 isolates were analyzed for antibiotic resistance genes. Four STEC and three isolates were phenotypically resistant to at least one of the antibiotics tested. Resistance genes (3″)-Ib, (3')-Ia, (6)-Id, , 2, (A), and A) were found in a human STEC ampicillin-resistant isolate. All isolates harbored fL, , and . Overall resistance in and STEC was low or middle. However, the high load of resistance genes found, even in susceptible isolates, suggests that these pathogens could contribute to the burden of antimicrobial resistance.
产志贺毒素大肠杆菌（STEC）是全球公认的人畜共患病原体。最近有报告称出现了对抗菌药物耐药的STEC分离株。为评估这些病原体中抗菌药物耐药性及相关基因的频率，我们研究了从不同来源本地分离得到的45株STEC和50株[此处原文缺失具体菌名]分离株。采用纸片扩散法进行抗菌药物敏感性试验，并对3株选定的STEC以及所有50株[此处原文缺失具体菌名]分离株的基因组序列进行抗生素耐药基因分析。4株STEC和3株[此处原文缺失具体菌名]分离株对至少一种测试抗生素表现出表型耐药。在一株对氨苄西林耐药的人源STEC分离株中发现了耐药基因（3″）-Ib、（3′）-Ia、（6）-Id、[此处原文缺失具体基因名称]、2、[此处原文缺失具体基因名称]（A）和A）。所有[此处原文缺失具体菌名]分离株都携带fL、[此处原文缺失具体基因名称]和[此处原文缺失具体基因名称]。[此处原文缺失具体菌名]和STEC的总体耐药性为低或中等。然而，即使在敏感分离株中也发现了高负荷的耐药基因，这表明这些病原体可能会加重抗菌药物耐药性的负担。
