Lai Ming-Chih, Peng Tsui-Yi, Tarn Woan-Yuh
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
FEBS J. 2009 Mar;276(6):1517-26. doi: 10.1111/j.1742-4658.2009.06894.x. Epub 2008 Feb 7.
Viruses take advantage of cellular machineries to facilitate their gene expression in the host. SR proteins, a superfamily of cellular precursor mRNA splicing factors, contain a domain consisting of repetitive arginine/serine dipeptides, termed the RS domain. The authentic RS domain or variants can also be found in some virus-encoded proteins. Viral proteins may act through their own RS domain or through interaction with cellular SR proteins to facilitate viral gene expression. Numerous lines of evidence indicate that cellular SR proteins are important for regulation of viral RNA splicing and participate in other steps of post-transcriptional viral gene expression control. Moreover, viral infection may alter the expression levels or modify the phosphorylation status of cellular SR proteins and thus perturb cellular precursor mRNA splicing. We review our current understanding of the interplay between virus and host in post-transcriptional regulation of gene expression via RS domain-containing proteins.
病毒利用细胞机制来促进其在宿主中的基因表达。SR蛋白是细胞前体mRNA剪接因子的一个超家族，包含一个由重复的精氨酸/丝氨酸二肽组成的结构域，称为RS结构域。在一些病毒编码的蛋白质中也能发现真正的RS结构域或变体。病毒蛋白可能通过其自身的RS结构域或通过与细胞SR蛋白相互作用来促进病毒基因表达。大量证据表明，细胞SR蛋白对病毒RNA剪接的调控很重要，并参与转录后病毒基因表达控制的其他步骤。此外，病毒感染可能会改变细胞SR蛋白的表达水平或改变其磷酸化状态，从而扰乱细胞前体mRNA的剪接。我们综述了目前对病毒与宿主之间通过含RS结构域的蛋白质在基因表达转录后调控中相互作用的理解。
