Giordani R, Moulin-Traffort J, Régli P
Centre de Biochimie et de Biologie Moléculaire, CNRS, Marseille, France.
Mycoses. 1991 Jan-Feb;34(1-2):67-73. doi: 10.1111/j.1439-0507.1991.tb00622.x.
Glycosidic activities have been examined in Candida albicans grown on medium culture containing latex sap (natural antifungal) or isoconazole (synthetic antifungal). The different types of utilized latex sap were those of Lactuca sativa (latex exuded from articulated laticifers) and Asclepias curassavica (latex flowing from non-articulated laticifers). The same enzyme assays were performed on C. albicans grown without antifungal compounds. Except for alpha-arabinosidase, all glycosidase activities were increased when C. albicans was grown in medium supplemented with L. sativa latex sap. The most stimulated activities were those of beta-fucosidase, alpha-galactosidase, alpha- and beta-glucosidase, alpha- and beta-mannosidase, acetyl-beta-glucosaminidase. The presence of A. curassavica latex sap in culture medium produced similar results: the most stimulated activities were those of alpha-mannosidase, alpha-galactosidase, acetyl-beta-glucosaminidase and beta-fucosidase. Electron microscope observations suggested a correlation between this stimulation of glycosidic activities and the fungal cell wall breakdown. For comparison the presence of isoconazole in culture medium yields no increase in glycosidic activities and no ultrastructural modification of fungal cell wall. The mode of action of latex saps in cell wall breakdown is discussed.
已对在含有乳胶汁（天然抗真菌剂）或异康唑（合成抗真菌剂）的培养基上生长的白色念珠菌的糖苷活性进行了检测。所使用的不同类型的乳胶汁分别是莴苣（从有节乳汁管分泌的乳胶）和马利筋（从无节乳汁管流出的乳胶）的乳胶汁。对在无抗真菌化合物的培养基上生长的白色念珠菌进行了相同的酶分析。除α -阿拉伯糖苷酶外，当白色念珠菌在添加了莴苣乳胶汁的培养基中生长时，所有糖苷酶活性均增加。受刺激最明显的活性是β -岩藻糖苷酶、α -半乳糖苷酶、α -和β -葡萄糖苷酶、α -和β -甘露糖苷酶、乙酰 -β -葡糖胺糖苷酶。培养基中存在马利筋乳胶汁也产生了类似结果：受刺激最明显的活性是α -甘露糖苷酶、α -半乳糖苷酶、乙酰 -β -葡糖胺糖苷酶和β -岩藻糖苷酶。电子显微镜观察表明，糖苷活性的这种刺激与真菌细胞壁的分解之间存在相关性。作为对照，培养基中存在异康唑不会使糖苷活性增加，也不会对真菌细胞壁产生超微结构改变。本文讨论了乳胶汁在细胞壁分解中的作用方式。
