Giordani R, Siepaio M, Moulin-Traffort J, Régli P
Centre de Biochimie et de Biologie Moléculaire, CNRS, Marseille, France.
Mycoses. 1991 Nov-Dec;34(11-12):469-77. doi: 10.1111/j.1439-0507.1991.tb00862.x.
Carica papaya latex inhibits the growth of Candida albicans. Latex proteins appear to be responsible for this antifungal effect. The minimum protein concentration for producing a complete inhibition was estimated to be about 138 micrograms ml-1. Exploration of different glycosidic activities shows that only alpha-D-mannosidase and N-acetyl-beta-D-glucosaminidase were present in latex in important levels and they were partially purified. The two enzymes show a limited inhibitory effect on yeast growth, alpha-D-mannosidase being more efficient than N-acetyl-beta-D-glucosaminidase. A mixture of the two enzymes showed a synergistic action on the inhibition of the yeast growth. Scanning and transmission electron microscopy observations showed a lack of polysaccharidic content on outermost layers of yeast cell walls when alpha-D-mannosidase was added to the culture medium. When C. albicans was cultured in medium supplemented with N-acetyl-beta-D-glucosaminidase a lack of polysaccharides was noted not only in the outermost layers of fungal cell wall but also in the inner layers. The potential utilization of latex glycosidases in combination with antifungals such as polyenes and azoles involving the formation of protoplasts is discussed.
番木瓜乳胶可抑制白色念珠菌的生长。乳胶蛋白似乎是这种抗真菌作用的原因。产生完全抑制作用的最低蛋白浓度估计约为138微克/毫升。对不同糖苷酶活性的研究表明，乳胶中仅α-D-甘露糖苷酶和N-乙酰-β-D-氨基葡萄糖苷酶含量较高，且已对其进行了部分纯化。这两种酶对酵母生长的抑制作用有限，α-D-甘露糖苷酶比N-乙酰-β-D-氨基葡萄糖苷酶更有效。这两种酶的混合物对酵母生长的抑制具有协同作用。扫描和透射电子显微镜观察表明，当向培养基中添加α-D-甘露糖苷酶时，酵母细胞壁最外层缺乏多糖成分。当白色念珠菌在添加了N-乙酰-β-D-氨基葡萄糖苷酶的培养基中培养时，不仅在真菌细胞壁的最外层，而且在内层都观察到多糖缺乏。文中讨论了乳胶糖苷酶与多烯类和唑类等抗真菌药物联合使用以形成原生质体的潜在用途。
