Suwanprateeb J, Sanngam R, Suvannapruk W, Panyathanmaporn T
National Metal and Materials Technology Center, National Science and Technology Development Agency, Ministry of Science and Technology, Klong, Klongluang, Pathumthani, Thailand.
J Mater Sci Mater Med. 2009 Jun;20(6):1281-9. doi: 10.1007/s10856-009-3697-1. Epub 2009 Feb 20.
In situ hydroxyapatite/apatite-wollastonite glass ceramic composite was fabricated by a three dimensional printing (3DP) technique and characterized. It was found that the as-fabricated mean green strength of the composite was 1.27 MPa which was sufficient for general handling. After varying sintering temperatures (1050-1300 degrees C) and times (1-10 h), it was found that sintering at 1300 degrees C for 3 h gave the greatest flexural modulus and strength, 34.10 GPa and 76.82 MPa respectively. This was associated with a decrease in porosity and increase in densification ability of the composite resulting from liquid phase sintering. Bioactivity tested by soaking in simulated body fluid (SBF) and In Vitro toxicity studies showed that 3DP hydroxyapatite/A-W glass ceramic composite was non-toxic and bioactive. A new calcium phosphate layer was observed on the surface of the composite after soaking in SBF for only 1 day while osteoblast cells were able to attach and attain normal morphology on the surface of the composite.
采用三维打印（3DP）技术制备了原位羟基磷灰石/磷灰石-硅灰石玻璃陶瓷复合材料并对其进行了表征。结果发现，所制备的复合材料的平均生坯强度为1.27MPa，这足以满足一般的操作要求。在改变烧结温度（1050-1300℃）和时间（1-10小时）后，发现1300℃烧结3小时可获得最大的弯曲模量和强度，分别为34.10GPa和76.82MPa。这与液相烧结导致的复合材料孔隙率降低和致密化能力增强有关。通过浸泡在模拟体液（SBF）中进行的生物活性测试和体外毒性研究表明，3DP羟基磷灰石/A-W玻璃陶瓷复合材料无毒且具有生物活性。在浸泡在SBF中仅1天后，在复合材料表面观察到一层新的磷酸钙层，而成骨细胞能够在复合材料表面附着并保持正常形态。
