提取红细胞酶以制备多聚血红蛋白-过氧化氢酶-超氧化物歧化酶。
Extraction of erythrocyte enzymes for the preparation of polyhemoglobin-catalase-superoxide dismutase.
作者信息
Gu Jingsong, Chang Thomas Ming Swi
机构信息
Artificial Cells & Organs Research Centre, Department of Physiology, McGill University, Montreal, Quebec, Canada.
出版信息
Artif Cells Blood Substit Immobil Biotechnol. 2009;37(2):69-77. doi: 10.1080/10731190902742240. Epub 2009 Feb 18.
Abstract
In sustained severe ischemia, reperfusion with oxygen carriers may result in ischemia-reperfusion injuries because of the release of damaging oxygen radicals. A nanobiotechnology-based polyhemogloin-calatase-superoxide dismutase can prevent this because the oxygen carrier, polyhemoglobin, is linked to antioxidant enzymes, catalase and superoxide dismutase. However, these antioxidant enzymes come from nonhuman sources and recombinant human enzymes are expensive. This paper describes our study on extracting these enzymes from red blood cells and analyzing the amount of enzymes needed for adequate protection from ischemia-reperfusion.
摘要
在持续性严重缺血的情况下，使用氧载体进行再灌注可能会因有害氧自由基的释放而导致缺血再灌注损伤。一种基于纳米生物技术的聚血红蛋白-过氧化氢酶-超氧化物歧化酶可以预防这种情况，因为氧载体聚血红蛋白与抗氧化酶过氧化氢酶和超氧化物歧化酶相连。然而，这些抗氧化酶来自非人类来源，且重组人源酶价格昂贵。本文描述了我们从红细胞中提取这些酶并分析充分保护免受缺血再灌注所需酶量的研究。
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