Yoshii Yuichi, Zhao Chunfeng, Schmelzer James D, Low Phillip A, An Kai-Nan, Amadio Peter C
Biomechanics Laboratory, Division of Orthopedic Research, Mayo Clinic, Rochester, MN 55905, USA.
Arch Phys Med Rehabil. 2009 Feb;90(2):333-9. doi: 10.1016/j.apmr.2008.07.028.
To investigate the effects of hypertonic dextrose injection on the subsynovial connective tissue (SSCT) in a rabbit model. We hypothesized that dextrose injection would induce proliferation of the SSCT, hinder median nerve conduction, and alter SSCT mechanical properties, similar to what is observed in patients with carpal tunnel syndrome (CTS).
Randomized, controlled prospective study.
Not applicable.
New Zealand white rabbits (N=28) weighing 4.0 to 4.5kg.
One forepaw was randomly injected with 0.1mL 10% dextrose solution. The contralateral paw was injected with a similar amount of 0.9% saline solution as a control. Animals were killed at 12 weeks after injection.
Animals were evaluated by electrophysiology (EP), mechanical testing, and histology. EP was evaluated by distal motor latency and amplitude. Shear force was evaluated when the middle digit flexor digitorum superficialis tendon was pulled out from the carpal tunnel. The ultimate tensile load and the energy absorption were also measured. Tissue for histology was evaluated qualitatively.
EP demonstrated significant prolongation of distal motor latency. The energy absorption and stiffness were also significantly increased in the dextrose group. Histologically, the dextrose group showed thickening of the collagen bundles and vascular proliferation within the SSCT compared with the saline group.
These results are consistent with the findings in patients with CTS and suggest that hypertonic dextrose injection has the potential to create a novel animal model in which to study the evolution of CTS.
在兔模型中研究高渗葡萄糖注射液对滑膜下结缔组织（SSCT）的影响。我们假设葡萄糖注射会诱导SSCT增殖，阻碍正中神经传导，并改变SSCT的力学性能，这与腕管综合征（CTS）患者中观察到的情况相似。
随机对照前瞻性研究。
不适用。
体重4.0至4.5千克的新西兰白兔（N = 28）。
随机向一只前爪注射0.1mL 10%葡萄糖溶液。对侧爪注射等量的0.9%生理盐水作为对照。注射后12周处死动物。
通过电生理学（EP）、力学测试和组织学对动物进行评估。通过远端运动潜伏期和波幅评估EP。从中指浅屈肌腱从腕管中拔出时评估剪切力。还测量了极限拉伸负荷和能量吸收。对组织学检查的组织进行定性评估。
EP显示远端运动潜伏期显著延长。葡萄糖组的能量吸收和刚度也显著增加。组织学上，与生理盐水组相比，葡萄糖组的SSCT内胶原束增厚且血管增生。
这些结果与CTS患者的研究结果一致，表明高渗葡萄糖注射有可能创建一种新型动物模型，用于研究CTS的演变。
