Angulo J F, Rouer E, Mazin A, Mattei M G, Tissier A, Horellou P, Benarous R, Devoret R
Groupe d'Etude Mutagénèse et Cancérogénèse, Laboratoire d'Enzymologie, CNRS, Gif-sur-Yvette, France.
Nucleic Acids Res. 1991 Oct 11;19(19):5117-23. doi: 10.1093/nar/19.19.5117.
We report the cloning of KIN17 cDNA, 1414 bp long with an ORF of 391 residues showing a zinc finger and nuclear localization signals. By recloning the cDNA into an appropriate vector, we produced kin17 protein in E. coli, purified it partially and shown that kin17 protein binds to double-stranded DNA. The KIN17 gene was localized by cytogenetic mapping in mouse chromosome 2, band A. Genomic sequences homologous to KIN17 cDNA were detected also in rat and human DNAs. KIN17 mRNA is highly expressed in rodent transformed AtT-20 neuroendocrine cells whereas it can be detected only in the total RNA of mouse embryos and various normal adult tissues by reverse transcription and PCR amplification. The mouse nuclear kin17 protein was identified by a local small structural similarity with E.coli recA protein. Kin17 and recA have only 39 amino acid residues in a region that might be involved in DNA-binding.
我们报道了KIN17 cDNA的克隆，其长度为1414 bp，开放阅读框含391个氨基酸残基，具有锌指结构和核定位信号。通过将该cDNA重新克隆到合适的载体中，我们在大肠杆菌中表达了kin17蛋白，并对其进行了部分纯化，结果表明kin17蛋白能与双链DNA结合。通过细胞遗传学定位，KIN17基因定位于小鼠2号染色体A带。在大鼠和人类DNA中也检测到了与KIN17 cDNA同源的基因组序列。KIN17 mRNA在啮齿动物转化的AtT-20神经内分泌细胞中高表达，而通过逆转录和PCR扩增，仅能在小鼠胚胎和各种正常成年组织的总RNA中检测到它。通过与大肠杆菌recA蛋白的局部小结构相似性鉴定出了小鼠核kin17蛋白。Kin17和recA在可能参与DNA结合的区域仅有39个氨基酸残基相同。
